Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATF2 antibody [E243] - BSA and Azide free (ab247240)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E243] to ATF2 - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB, Flow Cyt, IP, ChIP
  • Reacts with: Human

Overview

  • Product name

    Anti-ATF2 antibody [E243] - BSA and Azide free
    See all ATF2 primary antibodies

  • Description

    Rabbit monoclonal [E243] to ATF2 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody recognises ATF2, but does not cross react with other ATF family members. 

  • Tested applications

    Suitable for: ICC, IHC-P, WB, Flow Cyt, IP, ChIPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa cell lysate. IHC-P: Breast carcinoma tissue. Flow Cyt: HeLa cells. IP: HeLa cell lysate. ChIP: Jurkat cells. ICC: A549 cells

  • General notes

    ab247240 is the carrier-free version of ab32160 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247240 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-ATF2 antibody [E243] - BSA and Azide free (ab247240)
    Western blot - Anti-ATF2 antibody [E243] - BSA and Azide free (ab247240)
    Anti-ATF2 antibody [E243] - ChIP Grade (ab32160) at 1/10000 dilution + HeLa cell lysate

    Predicted band size: 54 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab32160, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody [E243] - BSA and Azide free (ab247240)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody [E243] - BSA and Azide free (ab247240)
    This data was developed using ab32160, the same antibody clone in a different buffer formulation.ab32160, at a dilution of 1/250, staining ATF2 in paraffin embedded breast carcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • ChIP - Anti-ATF2 antibody [E243] - BSA and Azide free (ab247240)
    ChIP - Anti-ATF2 antibody [E243] - BSA and Azide free (ab247240)
    This data was developed using ab32160, the same antibody clone in a different buffer formulation.Chromatin was prepared from Jurkat (TPA and Ionomycin treated or not) cells according to the Abcam X-ChIP protocol. Cells were fixed with 1% formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab32160 (red), and 20µl of protein A/G sepharose beads slurry (10µl of sepharose A beads + 10µl of sepharose G beads). 5μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
  • Immunocytochemistry - Anti-ATF2 antibody [E243] - BSA and Azide free (ab247240)
    Immunocytochemistry - Anti-ATF2 antibody [E243] - BSA and Azide free (ab247240)
    This data was developed using the same antibody clone in a different buffer formulation (ab32160).
    Immunocytochemistry analysis of A549 (human lung carcinoma epithelial cell) labeling ATF2 with purified ab32160 at 1/100 dilution (10 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.10 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
    Negative control: PBS instead of the primary antibody.
  • Immunoprecipitation - Anti-ATF2 antibody [E243] - BSA and Azide free (ab247240)
    Immunoprecipitation - Anti-ATF2 antibody [E243] - BSA and Azide free (ab247240)
    This data was developed using ab32160, the same antibody clone in a different buffer formulation.ab32160 (purified) at 1/60 dilution (20 µg/mL) immunoprecipitating ATF2 in HeLa whole cell lysate.
    Lane 3 (-): HeLa(Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µgab32160 & HeLa whole cell lysateRabbit monoclonal IgG (ab172730) instead of ab32160 in HeLa whole cell lysate
    For western blotting, ab32160 at 1/1000 dilution (2.284 µg/mL) and
    bbit TureBlot: Anti-Rabbit IgG HRP was used as the secondary antibody at 1/1500 dilution.
    Blocking and diluting buffer: 5% NFDM /TBST .
  • Flow Cytometry - Anti-ATF2 antibody [E243] - BSA and Azide free (ab247240)
    Flow Cytometry - Anti-ATF2 antibody [E243] - BSA and Azide free (ab247240)
    This data was developed using ab32160, the same antibody clone in a different buffer formulation.Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ATF2 (red) with ab32160 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
  • Anti-ATF2 antibody [E243] - BSA and Azide free (ab247240)
    Anti-ATF2 antibody [E243] - BSA and Azide free (ab247240)

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