Mouse IL-4 Matched Antibody Pair Kit (ab222274)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 0.98 pg/ml - 125 pg/ml
Overview
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Product name
Mouse IL-4 Matched Antibody Pair Kit
See all IL-4 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
0.1 pg/ml -
Range
0.98 pg/ml - 125 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse IL-4 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse IL-4.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab221833. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse IL-4 Capture Antibody 2 x 50µg 1 x 50µg Mouse IL-4 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse IL-4 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. -
Involvement in disease
Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. -
Sequence similarities
Belongs to the IL-4/IL-13 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- B cell growth factor 1
- B cell IgG differentiation factor
- B Cell Stimulatory Factor 1
see all -
Database links
- Entrez Gene: 16189 Mouse
- SwissProt: P07750 Mouse
- Unigene: 276360 Mouse
Images
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Mouse IL-4 standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse IL-4 Matched Antibody Pair Kit (ab222274)To learn more about the advantages of recombinant antibodies see here.
