Antibodies, Proteins, Kits and Reagents for Life Science

HRP Anti-ASS1 antibody [EPR12398] (ab209018)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
HRP Rabbit monoclonal [EPR12398] to ASS1
Suitable for: WB, IHC-P
Reacts with: Human
Conjugation: HRP

Product name

HRP Anti-ASS1 antibody [EPR12398]
See all ASS1 primary antibodies
Description

HRP Rabbit monoclonal [EPR12398] to ASS1
Host species

Rabbit
Conjugation

HRP
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human Liver lysate. IHC-P: FFPE normal human liver tissue sections.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage buffer

pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Monoclonal
Clone number

EPR12398
Isotype

IgG
Research areas

Western blot - HRP Anti-ASS1 antibody [EPR12398] (ab209018)

HRP Anti-ASS1 antibody [EPR12398] (ab209018) + Human liver lysate at 10 µg

Predicted band size: 47 kDa
Observed band size: 44 kDa
why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking buffer: 2% BSA

Gel type: MOPS
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-ASS1 antibody [EPR12398] (ab209018)

IHC image of ASS1 staining in a section of formalin-fixed paraffin-embedded normal human liver tissue*, performed on a Leica BOND^TM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab209018, 1/50 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
HRP Anti-ASS1 antibody [EPR12398] (ab209018)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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