Human H-FABP ELISA Kit (ab243682)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.4 pg/ml
- Range: 10.9 pg/ml - 700 pg/ml
- Sample type: Cerebral Spinal Fluid, Cit plasma, Hep Plasma, Milk, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cow, Human
Overview
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Product name
Human H-FABP ELISA Kit
See all H-FABP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 8 3.1% Inter-assay Sample n Mean SD CV% serum 2 12.4% -
Sample type
Milk, Serum, Tissue Extracts, Hep Plasma, Cit plasma, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
2.4 pg/ml -
Range
10.9 pg/ml - 700 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Milk 87 82% - 94% Serum 86 82% - 90% Tissue Extracts 106 104% - 108% Hep Plasma 91 89% - 94% Cit plasma 100 95% - 103% Cerebral Spinal Fluid 110 107% - 113% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Cow, Human -
Product overview
Human H-FABP ELISA Kit (ab243682) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of H-FABP protein in hep plasma, milk, serum, tissue extracts, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human H-FABP with 2.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human H-FABP Capture Antibody 1 x 600µl 10X Human H-FABP Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human H-FABP Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- 422 protein
- Cardiac FABP
- Cardiac Fatty Acid Binding Protein
see all -
Database links
- Entrez Gene: 281758 Cow
- Entrez Gene: 2170 Human
- Omim: 134651 Human
- SwissProt: P10790 Cow
- SwissProt: P05413 Human
- Unigene: 657242 Human
Images
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Other - Human H-FABP ELISA Kit (ab243682)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human H-FABP standard curve in Sample Diluent NS.The H-FABP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human H-FABP standard curve in 1X cell extraction buffer PTR.The H-FABP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native H-FABP in human serum, plasma and CSF samples.The concentrations of H-FABP were measured in duplicates, interpolated from the H-FABP standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (heparin) 25%, cerebrospinal fluid 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean H-FABP concentration was determined to be 1.93 ng/mL in serum, 2.05 ng/mL in plasma (citrate), 1.57 ng/mL in plasma (heparin), and 1.3 ng/mL in cerebrospinal fluid.
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Interpolated concentrations of native H-FABP in human milk sample.The concentrations of H-FABP were measured in duplicates, interpolated from the H-FABP standard curves and corrected for sample dilution. Undiluted samples are as follows: milk 1:50,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean H-FABP concentration was determined to be 4,273 ng/mL in milk 1:100,000.
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Interpolated concentrations of native H-FABP in human heart extract based on a 13 ng/mL extract load.The concentrations of H-FABP were measured in duplicate and interpolated from the H-FABP standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Serum from eight individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean H-FABP concentration was determined to be 1,635.8 pg/mL with a range of 873 – 2,940 pg/mL.
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Sandwich ELISA - Human H-FABP ELISA Kit (ab243682)To learn more about the advantages of recombinant antibodies see here.
