Monkey Cystatin C ELISA Kit (ab190542)
Key features and details
- Sensitivity: 0.3303 ng/ml
- Range: 0.125 ng/ml - 4 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Monkey
Overview
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Product name
Monkey Cystatin C ELISA Kit
See all Cystatin C kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum Inter-assay Sample n Mean SD CV% Serum -
Sample type
Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.3303 ng/ml -
Range
0.125 ng/ml - 4 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum > 85 % - % -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Monkey -
Product overview
Abcam’s Cystatin C Monkey ELISA Kit (ab190542) is for the measurement of Cystatin C in monkey serum and plasma.
In this assay the Cystatin C present in samples reacts with the anti-Cystatin C antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-Cystatin C, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Cystatin C in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Cystatin C in the test sample. The quantity of Cystatin C in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Notes
Cystatin C is a small cysteine proteinase inhibitor present in body fluids. Studies have shown Cystatin C levels to be directly correlated with the glomular filtration rate.
The calibrator used in this kit is of human origin.
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Platform
Microplate
Properties
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Components 1 x 96 tests 1X Diluent Concentrate 1 x 60ml 20X Wash Buffer Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Cystatin C Monkey Antibody coated microwells 1 x 96 tests Cystatin C Monkey Calibrator (lyophilized) 1 vial Cystatin C Monkey Detection Antibody 1 vial Cystatin C Monkey HRP Streptavidin Conjugate 1 vial Stop Solution 1 x 12ml -
Research areas
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Function
As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. -
Tissue specificity
Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland. -
Involvement in disease
Amyloidosis 6
Macular degeneration, age-related, 11 -
Sequence similarities
Belongs to the cystatin family. -
Post-translational
modificationsThe Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- AD 8
- AD8
- Amyloid angiopathy and cerebral hemorrhage
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Images
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Standard Curve
Representative standard curve using Cystatin Monkey ELISA kit (ab190542)