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Signal Transduction Protein Phosphorylation Tyrosine Kinases Receptor Tyrosine Kinases

Anti-IRR antibody [EP327Y] - BSA and Azide free (ab247288)

Anti-IRR antibody [EP327Y] - BSA and Azide free (ab247288)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP327Y] to IRR - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-IRR antibody [EP327Y] - BSA and Azide free
    See all IRR primary antibodies
  • Description

    Rabbit monoclonal [EP327Y] to IRR - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody recognises IRR related receptor. 

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247288 is the carrier-free version of ab40782.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP327Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • Insulin / Insulin-like
    • Neuroscience
    • Neurology process
    • Metabolism
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Heart disease

Images

  • Western blot - Anti-IRR antibody [EP327Y] - BSA and Azide free (ab247288)
    Western blot - Anti-IRR antibody [EP327Y] - BSA and Azide free (ab247288)
    All lanes : Anti-IRR antibody [EP327Y] (ab40782) at 1/2000 dilution

    Lane 1 : PC12 cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : SHSY5Y cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 80, 144 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 130 kDa, 37 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab40782, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRR antibody [EP327Y] - BSA and Azide free (ab247288)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRR antibody [EP327Y] - BSA and Azide free (ab247288)
    This data was developed using ab40782, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human kidney tissue using ab40782. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-IRR antibody [EP327Y] - BSA and Azide free (ab247288)
    Anti-IRR antibody [EP327Y] - BSA and Azide free (ab247288)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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