Anti-EGFR (phospho Y1148) antibody (ab5651)
Key features and details
- Rabbit polyclonal to EGFR (phospho Y1148)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EGFR (phospho Y1148) antibody
See all EGFR primary antibodies -
Description
Rabbit polyclonal to EGFR (phospho Y1148) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phosphopeptide (Human) derived from the region of EGFR that contains tyrosine 1148.
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Positive control
- A431 cells stimulated with EGF and/or NIH3T3 cells expressing human EGFR and stimulated with EGF.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody has been negatively preadsorbed using (i) a non phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated epidermal growth factor receptor (EGFR), and (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using an EGFR-derived peptide that is phosphorylated at tyrosine 1148. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EGFR (phospho Y1148) antibody (ab5651)Cell extracts prepared from NIH3T3 cells expressing EGFR and either not stimulated (-) or stimulated (+) with EGF were resolved by SDS-PAGE on a 10% Tris-glycine gel. The proteins were then transferred to nitrocellulose and incubated with 0.50
µ g/mL ab5651 antibody, following prior incubation with: no peptide (=None), the non-phosphorylated peptide corresponding to the phosphopeptide (=EGFR [pY1148] NP), or the phosphopeptide immunogen (=EGFR [pY1148] P). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase conjugate and bands were detected using the Tropix WesternStar method. The data presented here show that only the phosphopeptide corresponding to this site completely blocks the antibody signal, demonstrating the high specificity of the ab5651 antibody for this phosphorylated residue. Cell extracts prepared from NIH3T3 cells expressing EGFR and either not stimulated (-) or stimulated (+) with EGF were reso
