Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Friday March 05, 2021

Anti-Cannabinoid Receptor I (phospho S316) antibody [EPR2223(N)] (ab186428)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2223(N)] to Cannabinoid Receptor I (phospho S316)
Suitable for: WB, Flow Cyt
Reacts with: Human

Product name

Anti-Cannabinoid Receptor I (phospho S316) antibody [EPR2223(N)]
See all Cannabinoid Receptor I primary antibodies
Description

Rabbit monoclonal [EPR2223(N)] to Cannabinoid Receptor I (phospho S316)
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal brain lysate; SH-SY5Y cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR2223(N)
Isotype

IgG
Research areas

Western blot - Anti-Cannabinoid Receptor I (phospho S316) antibody [EPR2223(N)] (ab186428)

All lanes : Anti-Cannabinoid Receptor I (phospho S316) antibody [EPR2223(N)] (ab186428) at 1/1000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal brain lysate with Lambda phosphatase

Lysates/proteins at 1/10 dilution per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 53 kDa
Observed band size: 53 kDa
Additional bands at: 46 kDa (possible isoform)

Based on the sequence analysis, ab186428 recognizes three isoforms with the predicted MWs of 53KDa, 46KDa and 49KDa, respectively.
Flow Cytometry - Anti-Cannabinoid Receptor I (phospho S316) antibody [EPR2223(N)] (ab186428)

Flow cytometry analysis of Cannabinoid Receptor I (phospho S316) expression in 2% paraformaldehyde-fixed SH-SY5Y cells using ab186428 at 1/200 dilution (red) or a rabbit IgG isotype control (green), SH-SY5Y cells preincubated with Cannabinoid Receptor I (phospho S316) peptide (blue) or non-phosopho Cannabinoid Receptor I (phospho S316) peptide (orange). FITC-conjugated goat anti-rabbit IgG (1/150 dilution) was used as the secondary antibody.
Anti-Cannabinoid Receptor I (phospho S316) antibody [EPR2223(N)] (ab186428)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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