Anti-Cannabinoid Receptor I antibody (ab23703)
Key features and details
- Rabbit polyclonal to Cannabinoid Receptor I
- Suitable for: IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Cannabinoid Receptor I antibody
See all Cannabinoid Receptor I primary antibodies -
Description
Rabbit polyclonal to Cannabinoid Receptor I -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Rat
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Immunogen
Synthetic peptide:
MSVSTDTSAE AL
, corresponding to C terminal amino acids 461-472 of Human Cannabinoid Receptor I(Peptide available as ab50542.) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 50% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cannabinoid Receptor I antibody (ab23703)Anti-Cannabinoid Receptor I antibody (ab23703) + Human cerebellum at 30 µg
Predicted band size: 53 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Secondary antibody - anti-rabbit HRP (ab6721)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor I antibody (ab23703)Immunoperoxidase staining of CA region of rat hippocampus with the Cannabinoid Receptor I antibody (ab23703, 4ug/ml). the majority of the protein is localized in the pre-synaptic axons, shown as brown particulaes. A few neurons also show cytoplasm staining. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor I antibody (ab23703) Courtesy of an annonymous Abreviewab23703 staining Cannabinoid Receptor I in human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50 in PBS) for 8 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
