Anti-CRISPR-Cas9 antibody [8C1-F10] (ab210571)
![Anti-CRISPR-Cas9 antibody [8C1-F10] (ab210571)](https://www.abcam.com/ps/products/210/ab210571/Images/ab210571-271682-anti-crispr-cas9-antibody-8c1-f10-immunofluorescence.jpg "Anti-CRISPR-Cas9 antibody [8C1-F10] (ab210571)")
Key features and details
- Mouse monoclonal [8C1-F10] to CRISPR-Cas9
- Suitable for: WB, ICC/IF
- Reacts with: Streptococcus pyogenes
- Isotype: IgG2b
Overview
-
Product name
Anti-CRISPR-Cas9 antibody [8C1-F10]
See all CRISPR-Cas9 primary antibodies -
Description
Mouse monoclonal [8C1-F10] to CRISPR-Cas9 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Streptococcus pyogenes -
Immunogen
Recombinant fragment corresponding to Streptococcus pyogenes CRISPR-Cas9 (N terminal).
-
Positive control
- ICC-IF: NIH-3T3 Cas9-expressing cells
-
General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
8C1-F10 -
Isotype
IgG2b
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody [8C1-F10] (ab210571)
ab210571 stained in NIH3T3 cells. Untreated and Cas9 transfected cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab210571 at 10µg/ml and ab6046 (Rabbit polyclonal to beta tubulin) at 1ug/ml overnight at +4°C. The secondary antibodies were ab150177 (colored green) used at 1 ug/ml and ab150087 (pseudo-colored red) used at 2ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.
-
![Western blot - Anti-CRISPR-Cas9 antibody [8C1-F10] (ab210571)](https://www.abcam.com/ps/products/210/ab210571/Images/ab210571-270558-anti-crispr-cas9-antibody-8c1-f10-western-blot.jpg)
Western blot - Anti-CRISPR-Cas9 antibody [8C1-F10] (ab210571)All lanes : Anti-CRISPR-Cas9 antibody [8C1-F10] (Ab210571) at 1 µg/ml
Lane 1 : NIH3T3 non-transfected cell lysate
Lane 2 : NIH3T3 cells overexpressing CRISPR-Cas9
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 160 kDa
Exposure time: 10 seconds
