Estrogen Sulfotransferase overexpression 293T lysate (whole cell) (ab94289)
Overview
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Product name
Estrogen Sulfotransferase overexpression 293T lysate (whole cell)
See all Estrogen Sulfotransferase lysates -
General notes
ab94289 is a 293T cell transfected lysate in which Human Estrogen Sulfotransferase has been transiently over-expressed using a pCMV-Estrogen Sulfotransferase plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: May control the level of the estrogen receptor by sulfurylating free estradiol. Maximally sulfates beta-estradiol and estrone at concentrations of 20 nM. Also sulfates dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol and 1-naphthol, at significantly higher concentrations; however, cortisol, testosterone and dopamine are not sulfated. Tissue specificity: Liver, intestine and at lower level in the kidney. Similarity: Belongs to the sulfotransferase 1 family.
Images
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SDS-PAGE - Estrogen Sulfotransferase overexpression 293T lysate (whole cell) (ab94289)ab94289 at 15µg/lane on an SDS-PAGE gel.
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Western blot - Estrogen Sulfotransferase overexpression 293T lysate (whole cell) (ab94289)All lanes : Anti-Estrogen Sulfotransferase antibody (ab90441) at 1/500 dilution
Lane 1 :Estrogen Sulfotransferase overexpression 293T lysate (whole cell) (ab94289)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
