Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Laminin beta 1 antibody [EPR23265-32] (ab256380)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23265-32] to Laminin beta 1
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Laminin beta 1 antibody [EPR23265-32]
    See all Laminin beta 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23265-32] to Laminin beta 1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human stomach tissue lysate; Mouse stomach tissue lysate; Rat placenta, heart and stomach tissue lysates; C6, HepG2, SW480, NIH/3T3 and MEF whole cell lysates. ICC/IF: NIH/3T3 and HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Laminin beta 1 antibody [EPR23265-32] (ab256380)
    Western blot - Anti-Laminin beta 1 antibody [EPR23265-32] (ab256380)
    All lanes : Anti-Laminin beta 1 antibody [EPR23265-32] (ab256380) at 1/5000 dilution

    Lane 1 : Human stomach tissue lysate
    Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 3 : SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 198 kDa
    Observed band size: 250 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature (PMID: 11067943, 26705335). 

    Exposure time: Lane 1: 26 seconds; Lane 2: 10 seconds; Lane 3: 3 minutes.

  • Immunocytochemistry/ Immunofluorescence - Anti-Laminin beta 1 antibody [EPR23265-32] (ab256380)
    Immunocytochemistry/ Immunofluorescence - Anti-Laminin beta 1 antibody [EPR23265-32] (ab256380)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling Laminin beta 1 with ab256380 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cell line. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000  dilution.

  • Western blot - Anti-Laminin beta 1 antibody [EPR23265-32] (ab256380)
    Western blot - Anti-Laminin beta 1 antibody [EPR23265-32] (ab256380)
    All lanes : Anti-Laminin beta 1 antibody [EPR23265-32] (ab256380) at 1/5000 dilution

    Lane 1 : Mouse stomach tissue lysate
    Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 3 : MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 198 kDa
    Observed band size: 250 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST 

    Exposure time: 20 seconds

  • Immunocytochemistry/ Immunofluorescence - Anti-Laminin beta 1 antibody [EPR23265-32] (ab256380)
    Immunocytochemistry/ Immunofluorescence - Anti-Laminin beta 1 antibody [EPR23265-32] (ab256380)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Laminin dilution (Green). Confocal image showing cytoplasmic staining in NIH/3T3 cell line ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-Laminin beta 1 antibody [EPR23265-32] (ab256380)
    Western blot - Anti-Laminin beta 1 antibody [EPR23265-32] (ab256380)
    All lanes : Anti-Laminin beta 1 antibody [EPR23265-32] (ab256380) at 1/1000 dilution

    Lane 1 : Rat placenta tissue lysate
    Lane 2 : Rat heart tissue lysate
    Lane 3 : Rat stomach tissue lysate
    Lane 4 : C6 (rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 198 kDa
    Observed band size: 250 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST 

    Exposure time: Lanes 1-3: 70 seconds; Lane 4: 3 minutes.

  • Anti-Laminin beta 1 antibody [EPR23265-32] (ab256380)
    Anti-Laminin beta 1 antibody [EPR23265-32] (ab256380)

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