Anti-Laminin beta 1 antibody (ab69633)
Key features and details
- Rabbit polyclonal to Laminin beta 1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Laminin beta 1 antibody
See all Laminin beta 1 primary antibodies -
Description
Rabbit polyclonal to Laminin beta 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthesized peptide derived from the C-terminus of human Laminin beta 1.
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Positive control
- Hela cells, human liver carcinoma tissue and extracts from HepG2
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Laminin beta 1 antibody (ab69633) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells
Lane 2 : Extracts from HepG2 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 198 kDa
Observed band size: >170 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 130 kDa, 60 kDa, 67 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using ab69633 at 1:50 dilution.
Left image un-treated.Right image treated with immunizing peptide. -
Immunofluorescence analysis of HeLa cells using ab69633 at a 1:500 dilution.
Left image un-treated.Right image treated with immunizing peptide.