Anti-Kappa light chain antibody [SP148] (ab227654)
![Anti-Kappa light chain antibody [SP148] (ab227654)](https://www.abcam.com/ps/products/227/ab227654/Images/ab227654-8-AntiKappa-light-chain-antibody-SP148-IHC-Human-tonsil.jpg "Anti-Kappa light chain antibody [SP148] (ab227654)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP148] to Kappa light chain
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-Kappa light chain antibody [SP148]
See all Kappa light chain primary antibodies -
Description
Rabbit monoclonal [SP148] to Kappa light chain -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Other Immunogen Type corresponding to Human Kappa light chain. Free kappa light chain from pooled human IgG lambda myeloma sera.
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Positive control
- IHC-P: Human tonsil, colon, stomach, spleen, follicular lymphoma, and B-cell lymphoma tissue; WB: Human spleen lysate.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP148 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Kappa light chain with Purified ab227654 at 1/100 dilution (0.90 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Western blot - Anti-Kappa light chain antibody [SP148] (ab227654)](https://www.abcam.com/ps/products/227/ab227654/Images/ab227654-290345-anti-kappa-light-chain-antibody-sp148-western-blot.jpg)
Western blot - Anti-Kappa light chain antibody [SP148] (ab227654)Anti-Kappa light chain antibody [SP148] (ab227654) at 1/100 dilution + Human spleen lysate
Predicted band size: 25 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)](https://www.abcam.com/ps/products/227/ab227654/Images/ab227654-290339-anti-kappa-light-chain-antibody-sp148-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)Formalin-fixed, paraffin-embedded human tonsil tissue stained for Kappa light chain using ab227654 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)](https://www.abcam.com/ps/products/227/ab227654/Images/ab227654-290337-anti-kappa-light-chain-antibody-sp148-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)Formalin-fixed, paraffin-embedded human colon tissue stained for Kappa light chain using ab227654 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)](https://www.abcam.com/ps/products/227/ab227654/Images/ab227654-290336-anti-kappa-light-chain-antibody-sp148-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)Formalin-fixed, paraffin-embedded human stomach tissue stained for Kappa light chain using ab227654 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)](https://www.abcam.com/ps/products/227/ab227654/Images/ab227654-290335-anti-kappa-light-chain-antibody-sp148-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)Formalin-fixed, paraffin-embedded human follicular lymphoma tissue stained for Kappa light chain using ab227654 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)](https://www.abcam.com/ps/products/227/ab227654/Images/ab227654-290334-anti-kappa-light-chain-antibody-sp148-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)Formalin-fixed, paraffin-embedded human B-cell lymphoma tissue stained for Kappa light chain using ab227654 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)](https://www.abcam.com/ps/products/227/ab227654/Images/ab227654-290332-anti-kappa-light-chain-antibody-sp148-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)Formalin-fixed, paraffin-embedded human spleen tissue stained for Kappa light chain using ab227654 at 1/100 dilution in immunohistochemical analysis.
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![Anti-Kappa light chain antibody [SP148] (ab227654)](https://www.abcam.com/ps/products/227/ab227654/Images/ab227654-9-benefits-of-recombinant-antibodies.png)
Anti-Kappa light chain antibody [SP148] (ab227654)
