Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Kappa light chain antibody [SP148] (ab227654)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP148] to Kappa light chain
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Kappa light chain antibody [SP148]
    See all Kappa light chain primary antibodies

  • Description

    Rabbit monoclonal [SP148] to Kappa light chain

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Other Immunogen Type corresponding to Human Kappa light chain. Free kappa light chain from pooled human IgG lambda myeloma sera.

  • Positive control

    • IHC-P: Human tonsil, colon, stomach, spleen, follicular lymphoma, and B-cell lymphoma tissue; WB: Human spleen lysate.

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    Purified from TCS by protein A/G.

  • Clonality

    Monoclonal

  • Clone number

    SP148

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Kappa light chain with Purified ab227654 at 1/100 dilution (0.90 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-Kappa light chain antibody [SP148] (ab227654)
    Western blot - Anti-Kappa light chain antibody [SP148] (ab227654)
    Anti-Kappa light chain antibody [SP148] (ab227654) at 1/100 dilution + Human spleen lysate

    Predicted band size: 25 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for Kappa light chain using ab227654 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)

    Formalin-fixed, paraffin-embedded human colon tissue stained for Kappa light chain using ab227654 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)

    Formalin-fixed, paraffin-embedded human stomach tissue stained for Kappa light chain using ab227654 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)

    Formalin-fixed, paraffin-embedded human follicular lymphoma tissue stained for Kappa light chain using ab227654 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)

    Formalin-fixed, paraffin-embedded human B-cell lymphoma tissue stained for Kappa light chain using ab227654 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [SP148] (ab227654)

    Formalin-fixed, paraffin-embedded human spleen tissue stained for Kappa light chain using ab227654 at 1/100 dilution in immunohistochemical analysis.

  • Anti-Kappa light chain antibody [SP148] (ab227654)
    Anti-Kappa light chain antibody [SP148] (ab227654)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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