Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (ab235998)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1582Y] to GCDFP 15 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free
    See all GCDFP 15 primary antibodies

  • Description

    Rabbit monoclonal [EP1582Y] to GCDFP 15 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab155648)

  • Positive control

    • IHC-P: Cystic breast disease tissue.

  • General notes

    ab235998 is the carrier-free version of ab62363 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab235998 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (ab235998)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (ab235998)
    Immunohistochemical analysis of GCDFP 15 expression in paraffin embedded human breast carcinoma using 1/100-1/250 dilution of ab62363

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62363).

  • Immunocytochemistry/ Immunofluorescence - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (ab235998)
    Immunocytochemistry/ Immunofluorescence - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (ab235998)

    Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling GCDFP 15 with ab62363 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton-X. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291 Anti-Tubulin antibody (1/1000) followed by ab150120   AlexaFluor®594 Goat anti-Mouse secondary (1/1000). Nuclei were counterstained with DAPI (blue).

    For negative control 1, ab62363 at dilution of 1/100 was used followed by anti-mouse secondary antibody (ab150120) at 1/1000 dilution. For negative control 2, ab7291; 1/1000 was used followed by anti-rabbit secondary antibody (ab150077) at 1/1000 dilution.  

    Confocal image showing cytoplasmic staining on MCF7 cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62363).

  • Flow Cytometry - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (ab235998)
    Flow Cytometry - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (ab235998)

    Flow cytometry analysis of T47D (human mammary gland ductal carcinoma) (pink) with ab62363 at dilution of 1/20. The secondary antibody used was goat anti rabbit IgG (FITC) at dilution of 1/150. Cells were fixed with 2% paraformaldehyde. Isotype control antibody used was Rabbit monoclonal IgG (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62363).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (ab235998)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (ab235998)
    ab62363 showing positive staining in Salivary gland tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62363).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (ab235998)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (ab235998)
    ab62363 showing negative staining in Hepatocellular carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62363).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (ab235998)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (ab235998)
    ab62363 showing negative staining in Prostate hyperplasia tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62363).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (ab235998)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (ab235998)

    ab62363 showing positive staining in Cystic breast disease tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab62363).

  • Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (ab235998)
    Anti-GCDFP 15 antibody [EP1582Y] - BSA and Azide free (ab235998)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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