Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-GCDFP 15 antibody [EP1582Y] (ab62363)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1582Y] to GCDFP 15
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-GCDFP 15 antibody [EP1582Y]
See all GCDFP 15 primary antibodies
Description

Rabbit monoclonal [EP1582Y] to GCDFP 15
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human GCDFP 15 aa 1-100 (N terminal). The exact sequence is proprietary.
(Peptide available as ab155648)
Positive control
- T47D cell lysate, human breast carcinoma tissue
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP1582Y
Isotype

IgG
Research areas

Western blot - Anti-GCDFP 15 antibody [EP1582Y] (ab62363)

Anti-GCDFP 15 antibody [EP1582Y] (ab62363) at 1/2000 dilution + T47D cell lysate at 10 µg

Secondary
goat anti rabbit HRP at 1/2000 dilution

Predicted band size: 17 kDa
Observed band size: 17 kDa
Immunocytochemistry/ Immunofluorescence - Anti-GCDFP 15 antibody [EP1582Y] (ab62363)

Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling GCDFP 15 with ab62363 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton-X. ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291 Anti-Tubulin antibody (1/1000) followed by ab150120 AlexaFluor^®594 Goat anti-Mouse secondary (1/1000). Nuclei were counterstained with DAPI (blue).

For negative control 1, ab62363 at dilution of 1/100 was used followed by anti-mouse secondary antibody (ab150120) at 1/1000 dilution. For negative control 2, ab7291; 1/1000 was used followed by anti-rabbit secondary antibody (ab150077) at 1/1000 dilution.

Confocal image showing cytoplasmic staining on MCF7 cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (ab62363)

Immunohistochemical analysis of GCDFP 15 expression in paraffin embedded human breast carcinoma using 1/100-1/250 dilution of ab62363
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (ab62363)

ab62363 showing positive staining in Cystic breast disease tissue.
Flow Cytometry - Anti-GCDFP 15 antibody [EP1582Y] (ab62363)

Flow cytometry analysis of T47D (human mammary gland ductal carcinoma) (pink) with ab62363 at dilution of 1/20. The secondary antibody used was goat anti rabbit IgG (FITC) at dilution of 1/150. Cells were fixed with 2% paraformaldehyde. Isotype control antibody used was Rabbit monoclonal IgG (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (ab62363)

ab62363 showing positive staining in Salivary gland tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (ab62363)

ab62363 showing negative staining in Hepatocellular carcinoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [EP1582Y] (ab62363)

ab62363 showing negative staining in Prostate hyperplasia tissue.
Anti-GCDFP 15 antibody [EP1582Y] (ab62363)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com