Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP185] to GCDFP 15 - C-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-GCDFP 15 antibody [SP185] - C-terminal
    See all GCDFP 15 primary antibodies

  • Description

    Rabbit monoclonal [SP185] to GCDFP 15 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: T47D cell lysate IHC-P: Human breast carcinoma and breast ductal carcinoma tissues

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Cow: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling GCDFP 15 with ab183321 at 1/100 dilution (2.21 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human breast carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab183321 for 10 mins at room temperature.

  • Western blot - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)
    Western blot - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)
    Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321) at 1/400 dilution + T47D cell lysate

    Predicted band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling GCDFP with ab183321 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)

    Immunohistochemical analysis of paraffin-embedded Human breast ductal carcinoma tissue labeling GCDFP with ab183321 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)

    Immunohistochemical analysis of paraffin-embedded Human breast ductal carcinoma tissue labeling GCDFP with ab183321 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling GCDFP with ab183321 at 1/100 dilution.

  • Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)
    Anti-GCDFP 15 antibody [SP185] - C-terminal (ab183321)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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