Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Bcl10 antibody [EPR8587] (ab150380)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8587] to Bcl10
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-Bcl10 antibody [EPR8587]
See all Bcl10 primary antibodies
Description

Rabbit monoclonal [EPR8587] to Bcl10
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment corresponding to Human Bcl10 aa 1-250.
Positive control
- WB: Recombinant Human Bcl10 protein (ab124575); Ramos, HeLa, HuT-78 and Raji cell lysates. IHC-P: Human MALT and tonsil tissues.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR8587
Isotype

IgG
Research areas

Western blot - Anti-Bcl10 antibody [EPR8587] (ab150380)

All lanes : Anti-Bcl10 antibody [EPR8587] (ab150380) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : BCL10 knockout HeLa cell lysate
Lane 3 : Romas cell lysate
Lane 4 : A549 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 26 kDa
Observed band size: 32 kDa
why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab150380 observed at 32 kDa. Red - loading control, ab7291 observed at 52 kDa.

ab150380 Anti-Bcl10 antibody [EPR8587] was shown to specifically react with Bcl10 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261797 (knockout cell lysate ab257144) was used. Wild-type and Bcl10 knockout samples were subjected to SDS-PAGE. ab150380 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Bcl10 antibody [EPR8587] (ab150380)

All lanes : Anti-Bcl10 antibody [EPR8587] (ab150380) at 1/1000 dilution

Lane 1 : Ramos cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HuT-78 cell lysate
Lane 4 : Raji cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labeled goat anti-rabbit antibody at 1/2000 dilution

Predicted band size: 26 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [EPR8587] (ab150380)

Immunohistochemical analysis of paraffin embedded Human MALT tissue labeling Bcl10 with ab150380 at a 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody [EPR8587] (ab150380)

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Bcl10 with ab150380 at a 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Bcl10 antibody [EPR8587] (ab150380)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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