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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Cancer Drug resistance Topoisomerases

Anti-DNA Gyrase B antibody [7D3] (ab75586)

Anti-DNA Gyrase B antibody [7D3] (ab75586)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [7D3] to DNA Gyrase B
  • Suitable for: WB
  • Reacts with: Escherichia coli
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-DNA Gyrase B antibody [7D3]
    See all DNA Gyrase B primary antibodies
  • Description

    Mouse monoclonal [7D3] to DNA Gyrase B
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Escherichia coli
  • Immunogen

    Purified Escherichia coli DNA Gyrase B protein (over expressed from Escherichia coli)

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from tissue culture supernatant on a Protein A column using the low salt method. The antibody is purified to >95% purity as judged by SDS polyacrylamide gel electrophoresis.
  • Clonality

    Monoclonal
  • Clone number

    7D3
  • Isotype

    IgG2a
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Topoisomerases

Images

  • Western blot - Anti-DNA Gyrase B antibody [7D3] (ab75586)
    Western blot - Anti-DNA Gyrase B antibody [7D3] (ab75586)
    All lanes : Anti-DNA Gyrase B antibody [7D3] (ab75586) at 1/1000 dilution

    Lane 1 : E. coli cell lysate (B24 domain of DNA Gyrase B protein)
    Lane 2 : E. coli cell lysate (B43 domain of DNA Gyrase B protein)
    Lane 3 : E. coli cell lysate (whole DNA Gyrase B protein)

    Lysates/proteins at 200 µg per lane.

    Secondary
    All lanes : HRP labelled rabbit anti mouse IgGs at 1/5000 dilution

    Observed band size: 90 kDa
    why is the actual band size different from the predicted?

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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