Anti-DNA Gyrase B antibody [7D3] (ab75586)
Key features and details
- Mouse monoclonal [7D3] to DNA Gyrase B
- Suitable for: WB
- Reacts with: Escherichia coli
- Isotype: IgG2a
Overview
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Product name
Anti-DNA Gyrase B antibody [7D3]
See all DNA Gyrase B primary antibodies -
Description
Mouse monoclonal [7D3] to DNA Gyrase B -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Purified Escherichia coli DNA Gyrase B protein (over expressed from Escherichia coli)
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from tissue culture supernatant on a Protein A column using the low salt method. The antibody is purified to >95% purity as judged by SDS polyacrylamide gel electrophoresis. -
Clonality
Monoclonal -
Clone number
7D3 -
Isotype
IgG2a -
Research areas
Images
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All lanes : Anti-DNA Gyrase B antibody [7D3] (ab75586) at 1/1000 dilution
Lane 1 : E. coli cell lysate (B24 domain of DNA Gyrase B protein)
Lane 2 : E. coli cell lysate (B43 domain of DNA Gyrase B protein)
Lane 3 : E. coli cell lysate (whole DNA Gyrase B protein)
Lysates/proteins at 200 µg per lane.
Secondary
All lanes : HRP labelled rabbit anti mouse IgGs at 1/5000 dilution
Observed band size: 90 kDa why is the actual band size different from the predicted?