beta-Lapachone, Topoisomerase I inhibitor (ab141097)
Key features and details
- Topoisomerase I inhibitor
- CAS Number: 4707-32-8
- Purity: > 98%
- Soluble in ethanol to 50 mM and in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
beta-Lapachone, Topoisomerase I inhibitor -
Description
Topoisomerase I inhibitor -
Biological description
Topoisomerase I inhibitor. Inhibits topoisomerase I induced DNA cleavage. Induces apoptosis via a p53 independent mechanism. Shows antitumor, antiviral, antibacterial, antifungal and wound healing activities.
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Purity
> 98% -
CAS Number
4707-32-8 -
Chemical structure
Images
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Functional Studies - beta-Lapachone, Topoisomerase I inhibitor (ab141097)ab18209 staining p21 in serum starved PC-3 cells treated with ß-lapachone (ab141097), by ICC/IF. Increase of p21 nuclear expression correlates with increased concentration of ß-lapachone, as described in literature.
The cells were incubated at 37°C for 6 hours in media containing different concentrations of ab141097 (ß-lapachone) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab18209 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
