Anti-DNA Gyrase B antibody (ab75596)
Key features and details
- Rabbit polyclonal to DNA Gyrase B
- Suitable for: WB
- Reacts with: Escherichia coli, Staphylococcus aureus
- Isotype: IgG
Overview
-
Product name
Anti-DNA Gyrase B antibody
See all DNA Gyrase B primary antibodies -
Description
Rabbit polyclonal to DNA Gyrase B -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Escherichia coli, Staphylococcus aureus -
Immunogen
Purified E. coli Gyr B protein (over expressed from E. coli)
-
Positive control
- E. coli cell lysate
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
This antibody is isolated from rabbit blood and further purified on a Protein A column using the low salt method. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-DNA Gyrase B antibody (ab75596)All lanes : Anti-DNA Gyrase B antibody (ab75596) at 1/1000 dilution
Lane 1 : E. coli cell lysate (Gyrase A, whole protein) at 0.1 µg
Lane 2 : E. coli cell lysate (Gyrase A, whole protein) at 0.5 µg
Lane 3 : E. coli cell lysate (Gyrase A, whole protein) at 1 µg
Lane 4 : E. coli cell lysate (Gyrase B, whole protein) at 0.1 µg
Lane 5 : E. coli cell lysate (Gyrase B, whole protein) at 0.5 µg
Lane 6 : E. coli cell lysate (Gyrase B, whole protein) at 1 µg
Lane 7 : E. coli cell lysate (Gyrase B, B43 domain) at 0.1 µg
Lane 8 : E. coli cell lysate (Gyrase A, A59 domain) at 0.1 µg
Secondary
All lanes : goat anti rabbit Igs at 1/5000 dilution
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
