Anti-Bcl10 antibody (ab227206)
Key features and details
- Rabbit polyclonal to Bcl10
- Suitable for: IHC-P, WB, ICC/IF, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Bcl10 antibody
See all Bcl10 primary antibodies -
Description
Rabbit polyclonal to Bcl10 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Rhesus monkey -
Immunogen
Recombinant fragment within Human Bcl10 (internal sequence). The exact sequence is proprietary.
Database link: O95999 -
Positive control
- WB: HeLa cell extract; Raji, Neuro-2a, GL261 and C8D30 whole cell lysates. IP: Raji whole cell extract. IHC-P: Human gastric cancer tissue. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Bcl10 antibody (ab227206) at 1/2500 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : Bcl10 Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 26 kDa
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All lanes : Anti-Bcl10 antibody (ab227206) at 1/2500 dilution
Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 2 : Bcl10 knockout HeLa cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 26 kDa12% SDS-PAGE gel.
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Anti-Bcl10 antibody (ab227206) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Predicted band size: 26 kDa12% SDS PAGE gel.
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All lanes : Anti-Bcl10 antibody (ab227206) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 2 : GL261 whole cell lysate
Lane 3 : C8D30 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa12 % SDS-PAGE gel.
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Bcl10 was immunoprecipitated from Raji (human Burkitt's lymphoma cell line) whole cell extract with 4 μg ab227206. Western blot was performed from the immunoprecipitate using ab227206 at 1/500 dilution. Anti-rabbit IgG was used as a secondary reagent
Lane 1: Raji whole cell extract 40 μg (Input).
Lane 2: Control IP in Raji whole cell extract with 4 μg of preimmune Rabbit IgG.
Lane 3: ab227206 IP in Raji whole cell extract.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl10 antibody (ab227206)
Paraffin-embedded human gastric cancer tissue stained for Bcl10 using ab227206 at 1/500 dilution in immunohistochemical analysis.
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2% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Bcl10 (green) using ab227206 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.