Recombinant human PRMT6 protein (ab196434)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 45% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human PRMT6 protein
See all PRMT6 proteins and peptides -
Biological activity
Specific Activity 0.015 pmol/min/µg
Assay Conditions: 50 µl reaction mix (20 mM phosphate buffer pH 7.4, 20 µM S-adenosylmethionine, and 1-5 ng methyltransferase PRMT6) is added to microwells coated with histone substrate. Incubate at 30°C for 2 hr. Add antibody against methylated Arg residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence. -
Purity
>= 45 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
SQPKKRKLESGGGGEGGEGTEEEDGAEREAALERPRRTKRERDQLYYECY SDVSVHEEMIADRVRTDAYRLGILRNWAALRGKTVLDVGAGTGILSIFCA QAGARRVYAVEASAIWQQAREVVRFNGLEDRVHVLPGPVETVELPEQVDA IVSEWMGYGLLHESMLSSVLHARTKWLKEGGLLLPASAELFIAPISDQML EWRLGFWSQVKQHYGVDMSCLEGFATRCLMGHSEIVVQGLSGEDVLARPQ RFAQLELSRAGLEQELEAGVGGRFRCSCYGSAPMHGFAIWFQVTFPGGES EKPLVLSTSPFHPATHWKQALLYLNEPVQVEQDTDVSGEITLLPSRDNPR RLRVLLRYKVGDQEEKTKDFAMED -
Predicted molecular weight
43 kDa including tags -
Amino acids
2 to 375 -
Tags
His tag N-Terminus -
Additional sequence information
Genbank accession no.: NM_018137
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 1.36% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human PRMT6 protein (ab196434)
SDS-PAGE analysis of 3 μg of ab196434 on a 10% SDS-PAGE gel stained with Coomassie.
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SDS-PAGE - Recombinant human PRMT6 protein (ab196434)SDS-PAGE analysis of 2 μg of ab196434 on a 4-20% SDS-PAGE gel stained with Coomassie.
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Functional Studies - Recombinant human PRMT6 protein (ab196434)Specific activity of ab196434.