Anti-PABPN1 antibody [EP3001Y] (ab81224)
![Anti-PABPN1 antibody [EP3001Y] (ab81224)](https://www.abcam.com/ps/products/81/ab81224/Images/PABP2-Primary-antibodies-ab81224-3.jpg "Anti-PABPN1 antibody [EP3001Y] (ab81224)")
Key features and details
- Rabbit monoclonal [EP3001Y] to PABPN1
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PABPN1 antibody [EP3001Y]
See all PABPN1 primary antibodies -
Description
Rabbit monoclonal [EP3001Y] to PABPN1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow
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Immunogen
Synthetic peptide within Human PABPN1 aa 150-250. The exact sequence is proprietary.
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Positive control
- WB: T47D, SKBR3, Ramos and Raji cell lysates; IHC: urinary bladder transitional cell carcinoma.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as PABP2
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP3001Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PABPN1 antibody [EP3001Y] (ab81224)All lanes : Anti-PABPN1 antibody [EP3001Y] (ab81224) at 1/20000 dilution
Lane 1 : T47D cell
lysates
Lane 2 : SKBR3 cell
lysates
Lane 3 : Ramos cell
lysates
Lane 4 : Raji cell
lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PABPN1 antibody [EP3001Y] (ab81224)](https://www.abcam.com/ps/products/81/ab81224/Images/PABP2-Primary-antibodies-ab81224-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PABPN1 antibody [EP3001Y] (ab81224)Immunohistochemistry staining of paraffin-embedded urinary bladder transitional cell carcinoma tissue, using 1/100 ab81224
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-PABPN1 antibody [EP3001Y] (ab81224)](https://www.abcam.com/ps/products/81/ab81224/Images/PABP2-Primary-antibodies-ab81224-5.jpg)
Flow Cytometry - Anti-PABPN1 antibody [EP3001Y] (ab81224)Overlay histogram showing HeLa cells stained with ab81224 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81224, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
