Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NSDHL antibody [EPR14490] (ab190353)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14490] to NSDHL
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-NSDHL antibody [EPR14490]
    See all NSDHL primary antibodies

  • Description

    Rabbit monoclonal [EPR14490] to NSDHL

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T, A431, HeLa and Human fetal brain lysates. IHC-P: Human liver and Human adenocarcinoma of endometrium tissue. ICC: U87-MG cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NSDHL antibody [EPR14490] (ab190353)
    Western blot - Anti-NSDHL antibody [EPR14490] (ab190353)
    All lanes : Anti-NSDHL antibody [EPR14490] (ab190353) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : NSDHL knockout HEK293T cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 42 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab190353 observed at 38 kDa. Red - loading control ab7291 observed at 50 kDa.

     ab190353 Anti-NSDHL antibody [EPR14490]  was shown to specifically react with NSDHL in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266682 (knockout cell lysate ab258082) was used. Wild-type and NSDHL knockout samples were subjected to SDS-PAGE. ab190353 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSDHL antibody [EPR14490] (ab190353)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSDHL antibody [EPR14490] (ab190353)

    Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of endometrium tissue labeling NSDHL with ab190353 at 1/100 dilution. Secondary antibody HRP Polymer for Rabbit IgG prediluted. Counter stain Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSDHL antibody [EPR14490] (ab190353)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSDHL antibody [EPR14490] (ab190353)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling NSDHL with ab190353 at 1/100 dilution. Secondary antibody HRP Polymer for Rabbit IgG prediluted. Counter stain Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-NSDHL antibody [EPR14490] (ab190353)
    Immunocytochemistry/ Immunofluorescence - Anti-NSDHL antibody [EPR14490] (ab190353)

    Immunofluorescence analysis of U87-MG cells, labeling NSDHL (red) with ab190353 at 1/50 dilution. Alexa Fluor®555-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

  • Western blot - Anti-NSDHL antibody [EPR14490] (ab190353)
    Western blot - Anti-NSDHL antibody [EPR14490] (ab190353)
    All lanes : Anti-NSDHL antibody [EPR14490] (ab190353) at 1/10000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 38 kDa why is the actual band size different from the predicted?

  • Anti-NSDHL antibody [EPR14490] (ab190353)
    Anti-NSDHL antibody [EPR14490] (ab190353)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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