Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µg 307 566 ₸ 10 µg 103 862 ₸

Order now and get it on Wednesday March 10, 2021

Anti-CD44 antibody [Hermes-3] (ab254530)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [Hermes-3] to CD44
Suitable for: ELISA, WB, IHC-P
Reacts with: Human, Recombinant fragment

Product name

Anti-CD44 antibody [Hermes-3]
See all CD44 primary antibodies
Description

Mouse monoclonal [Hermes-3] to CD44
Host species

Mouse
Tested Applications & Species

Application Species
IHC-P
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Recombinant fragment corresponding to Human CD44.

Run BLAST with Run BLAST with
Positive control
- WB: A549 and HeLa whole cell lysate. IHC-P: Human skin tissue. Human bladder carcinoma tissue.
General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
IHC-P	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

Hermes-3
Isotype

IgG2a
Research areas

Western blot - Anti-CD44 antibody [Hermes-3] (ab254530)

All lanes : Anti-CD44 antibody [Hermes-3] (ab254530) at 0.536 µg/ml

Lane 1 : A549 (human lung carcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 81 kDa

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [Hermes-3] (ab254530)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling CD44 with ab254530 at 0.107µg/ml, followed by ready to use secondary. Membranous staining on human skin is observed. The section was incubated with ab254530 for 5 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [Hermes-3] (ab254530)

Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue labeling CD44 with ab254530 at 0.107µg/ml, followed by ready to use secondary. Membranous staining on human bladder carcinoma is observed. The section was incubated with ab254530 for 5 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
ELISA - Anti-CD44 antibody [Hermes-3] (ab254530)

ELISA - Anti-CD44 antibody [Hermes-3] (ab254530).

Antigen: Human CD44.

ab254530 used at 100-1000µg/ml.

Secondary is an Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) used at a 1/1000 dilution.
Anti-CD44 antibody [Hermes-3] (ab254530)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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