Anti-SLC2A4RG antibody (ab86756)
Key features and details
- Rabbit polyclonal to SLC2A4RG
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SLC2A4RG antibody -
Description
Rabbit polyclonal to SLC2A4RG -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human SLC2A4RG aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available asab136159) -
Positive control
- This antibody gave a positive signal in Human Skeletal Muscle tissue lysate. This antibody gave a positive result in IF in the following Methanol fixed cell line: MCF-7
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86756 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanAll applications HorseChimpanzeeMacaque monkeyGorillaApplication Abreviews Notes ICC/IF Use a concentration of 5 µg/ml.WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).Notes ICC/IF
Use a concentration of 5 µg/ml.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).Target
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Function
Transcription factor involved in SLC2A4 and HD gene transactivation. Binds to the consensus sequence 5'-GCCGGCG-3'. -
Tissue specificity
According to PubMed:14630949, expressed in heart, skeletal muscle, liver, kidney and pancreas; undetectable in lung, placenta or brain. According to PubMed:14625278, ubiquitously expressed, with lowest expression in brain and ileum. -
Sequence similarities
Contains 1 C2H2-type zinc finger. -
Cellular localization
Cytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 56731 Human
- Omim: 609493 Human
- SwissProt: Q9NR83 Human
- Unigene: 435126 Human
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Alternative names
- GEF antibody
- GLUT4 enhancer factor antibody
- HDBP 1 antibody
see all
Images
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Western blot - Anti-SLC2A4RG antibody (ab86756)Anti-SLC2A4RG antibody (ab86756) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 41 kDa
Additional bands at: 24 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-SLC2A4RG antibody (ab86756)ICC/IF image of ab86756 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab86756 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde fixed (4%, 10min) HeLa, Hek293, HepG2, and MCF-7 cell types, and in Methanol fixed (100%, 5min) Hek293 cell types.
Protocols
Datasheets and documents
References (0)
ab86756 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-SLC2A4RG antibody (ab86756)Anti-SLC2A4RG antibody (ab86756) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 41 kDa
Additional bands at: 24 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-SLC2A4RG antibody (ab86756)
ICC/IF image of ab86756 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab86756 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde fixed (4%, 10min) HeLa, Hek293, HepG2, and MCF-7 cell types, and in Methanol fixed (100%, 5min) Hek293 cell types.
