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Signal Transduction Metabolism Energy Metabolism

Anti-PKM2 antibody (ab137852)

Price and availability

284 784 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-PKM2 antibody (ab137852)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to PKM2
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PKM2 antibody
    See all PKM2 primary antibodies
  • Description

    Rabbit polyclonal to PKM2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human PKM2 aa 1-237. The exact sequence is proprietary. The exact immunogen sequence is within the center region.
    Database link: P14618

  • Positive control

    • WB: 293T, A431, HeLa. IHC-P: DLD1 xenograft tissue; human lung tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • Other
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Images

  • Western blot - Anti-PKM2 antibody (ab137852)
    Western blot - Anti-PKM2 antibody (ab137852)
    All lanes : Anti-PKM2 antibody (ab137852) at 1/500 dilution

    Lane 1 : Non-transfected (–) 293T whole cell extracts
    Lane 2 : 293T whole cell extracts transfected (+) with PKM shRNA

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution

    Predicted band size: 58 kDa



    7.5% SDS-PAGE.

    Running conditions: 80V, 15min; 140V, 40 minutes.

    Transfer condition: Semi-dry, 18 V, 60 min (NC membrane).

    Blocking condition: 5% non-fat milk in TBST, RT, 60 minutes.

    Primary antibody incubation: 4? , overnight.

    Washing condition: 5 ml TBST, 4 x 5 minutes. 

    Exposure: chemiluminescent substrate for the detection of HRP-conjugated antibody

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKM2 antibody (ab137852)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKM2 antibody (ab137852)

    Paraffin-embedded human lung tissue stained for PKM2 using ab137852 at 1/500 dilution (4°C overnight) in immunohistochemical analysis.

    Secondary antibody: ABC HRP Kit (Rabbit IgG) 1/200 at room temperature for 30 minutes. 

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 minutes. (Cuisinart Electric Pressure Cooker, choose "high pressure").

    Endogenous peroxide blocking: 3% H2O2, RT, 30minutes.

    Blocking condition: 1.5% goat serum (dilute goat serum by 1xPBS), RT, 30 minutes.

    Washing condition: PBS, 2 x 5 minutes.

    Chromogen system: DAB. 

     

  • Western blot - Anti-PKM2 antibody (ab137852)
    Western blot - Anti-PKM2 antibody (ab137852)
    All lanes : Anti-PKM2 antibody (ab137852) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate
    Lane 4 : HeLa S3 whole cell lysate
    Lane 5 : Hep G2 whole cell lysate
    Lane 6 : MOLT4 whole cell lysate
    Lane 7 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution

    Predicted band size: 58 kDa



    10% SDS-PAGE.

    Running conditions: 80V, 15min; 140V, 40 minutes.

    Transfer condition: Semi-dry, 18 V, 60 min (NC membrane).

    Blocking condition: 5% non-fat milk in TBST, RT, 60 minutes.

    Primary antibody incubation: 4? , overnight.

    Washing condition: 5 ml TBST, 4 x 5 minutes. 

    Exposure: enhanced ECL

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKM2 antibody (ab137852)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKM2 antibody (ab137852)

    Immunohistochemical analysis of paraffin-embedded PKM2 DLD1 xenograft tissue labelling PKM2 with ab137852 at 1/100 dilution.

  • Western blot - Anti-PKM2 antibody (ab137852)
    Western blot - Anti-PKM2 antibody (ab137852)
    Anti-PKM2 antibody (ab137852) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 58 kDa



    7.5% SDS PAGE
  • Western blot - Anti-PKM2 antibody (ab137852)
    Western blot - Anti-PKM2 antibody (ab137852)
    Anti-PKM2 antibody (ab137852) at 1/1000 dilution + Rat brain lysate/extract at 50 µg

    Predicted band size: 58 kDa



    7.5 % SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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