Anti-PKM2 antibody (ab137852)
Key features and details
- Rabbit polyclonal to PKM2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PKM2 antibody
See all PKM2 primary antibodies -
Description
Rabbit polyclonal to PKM2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human PKM2 aa 1-237. The exact sequence is proprietary. The exact immunogen sequence is within the center region.
Database link: P14618 -
Positive control
- WB: 293T, A431, HeLa. IHC-P: DLD1 xenograft tissue; human lung tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PKM2 antibody (ab137852) at 1/500 dilution
Lane 1 : Non-transfected (–) 293T whole cell extracts
Lane 2 : 293T whole cell extracts transfected (+) with PKM shRNA
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution
Predicted band size: 58 kDa7.5% SDS-PAGE.
Running conditions: 80V, 15min; 140V, 40 minutes.
Transfer condition: Semi-dry, 18 V, 60 min (NC membrane).
Blocking condition: 5% non-fat milk in TBST, RT, 60 minutes.
Primary antibody incubation: 4? , overnight.
Washing condition: 5 ml TBST, 4 x 5 minutes.
Exposure: chemiluminescent substrate for the detection of HRP-conjugated antibody
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Paraffin-embedded human lung tissue stained for PKM2 using ab137852 at 1/500 dilution (4°C overnight) in immunohistochemical analysis.
Secondary antibody: ABC HRP Kit (Rabbit IgG) 1/200 at room temperature for 30 minutes.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 minutes. (Cuisinart Electric Pressure Cooker, choose "high pressure").
Endogenous peroxide blocking: 3% H2O2, RT, 30minutes.
Blocking condition: 1.5% goat serum (dilute goat serum by 1xPBS), RT, 30 minutes.
Washing condition: PBS, 2 x 5 minutes.
Chromogen system: DAB.
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All lanes : Anti-PKM2 antibody (ab137852) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa S3 whole cell lysate
Lane 5 : Hep G2 whole cell lysate
Lane 6 : MOLT4 whole cell lysate
Lane 7 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution
Predicted band size: 58 kDa10% SDS-PAGE.
Running conditions: 80V, 15min; 140V, 40 minutes.
Transfer condition: Semi-dry, 18 V, 60 min (NC membrane).
Blocking condition: 5% non-fat milk in TBST, RT, 60 minutes.
Primary antibody incubation: 4? , overnight.
Washing condition: 5 ml TBST, 4 x 5 minutes.
Exposure: enhanced ECL
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Immunohistochemical analysis of paraffin-embedded PKM2 DLD1 xenograft tissue labelling PKM2 with ab137852 at 1/100 dilution.
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Anti-PKM2 antibody (ab137852) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 58 kDa
7.5% SDS PAGE -
Anti-PKM2 antibody (ab137852) at 1/1000 dilution + Rat brain lysate/extract at 50 µg
Predicted band size: 58 kDa
7.5 % SDS-PAGE