Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR075G] to PCNA
Suitable for: ICC, IHC-P, WB
Reacts with: Mouse, Human

Product name

Anti-PCNA antibody [BLR075G]
See all PCNA primary antibodies
Description

Rabbit monoclonal [BLR075G] to PCNA
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human PCNA aa 225-261. The exact sequence is proprietary. NP_002583.1
Database link: P12004
Positive control
- WB: A549, Jurkat, HEK-293T, RKO, K562, MCF7, HeLa and U-2 OS whole cell lysate. IHC-P: Human prostate carcinoma and ovarian carcinoma tissue. ICC: OVCAR-3 cells.
General notes

This product is sold under License from Bethyl Laboratories, Inc

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

BLR075G
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab265609)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling PCNA with ab265609 at 1/250 dilution. Detection: DAB.
Western blot - Anti-PCNA antibody (ab265609)

All lanes : Anti-PCNA antibody [BLR075G] (ab265609) at 1/1000 dilution

Lane 1 : A549 whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : HEK-293T whole cell lysate
Lane 4 : RKO whole cell lysate
Lane 5 : K562 whole cell lysate
Lane 6 : MCF7 whole cell lysate
Lane 7 : HeLa whole cell lysate
Lane 8 : U-2 OS whole cell lysate
Lane 9 : TCMK whole cell lysate
Lane 10 : NIH/3T3 whole cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG

Predicted band size: 29 kDa

Exposure time: 30 seconds

Cells prepared using NETN lysis buffer.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab265609)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling PCNA with ab265609 at 1/250 dilution. Detection: DAB.
Immunocytochemistry - Anti-PCNA antibody (ab265609)

Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-3 cells labeling PCNA with ab265609 at 1/250 dilution. Detection: DAB.
Anti-PCNA antibody [BLR075G] (ab265609)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com