Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PCNA antibody [EPR3822] - BSA and Azide free (ab247595)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3822] to PCNA - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PCNA antibody [EPR3822] - BSA and Azide free
    See all PCNA primary antibodies

  • Description

    Rabbit monoclonal [EPR3822] to PCNA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC or IP

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247595 is the carrier-free version of ab92729 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247595 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3822] - BSA and Azide free (ab247595)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3822] - BSA and Azide free (ab247595)
    This data was developed using ab92729, the same antibody clone in a different buffer formulation.ab92729, at 1/50 dilution, staining PCNA in paraffin embedded Human colonic adenocarcinoma tissue by Immunohistochemisty. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3822] - BSA and Azide free (ab247595)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3822] - BSA and Azide free (ab247595)
    This data was developed using ab92729, the same antibody clone in a different buffer formulation.ab92729, at 1/50 dilution, staining PCNA in paraffin embedded Human tonsil tissue by Immunohistochemisty. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Western blot - Anti-PCNA antibody [EPR3822] - BSA and Azide free (ab247595)
    Western blot - Anti-PCNA antibody [EPR3822] - BSA and Azide free (ab247595)
    All lanes : Anti-PCNA antibody [EPR3822] (ab92729) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 29 kDa



    This data was developed using ab92729, the same antibody clone in a different buffer formulation.

     

  • Anti-PCNA antibody [EPR3822] - BSA and Azide free (ab247595)
    Anti-PCNA antibody [EPR3822] - BSA and Azide free (ab247595)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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