Antibodies, Proteins, Kits and Reagents for Life Science

526 012 ₸ Product size

100 µg 526 012 ₸ 1 mg 4 690 ₸

Order now and get it on Tuesday March 09, 2021

Anti-PCNA antibody [EPR3822] - BSA and Azide free (ab247595)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3822] to PCNA - BSA and Azide free
Suitable for: IHC-P, WB
Reacts with: Mouse, Human

Product name

Anti-PCNA antibody [EPR3822] - BSA and Azide free
See all PCNA primary antibodies
Description

Rabbit monoclonal [EPR3822] to PCNA - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IHC-P, WBmore details
Unsuitable for: ICC or IP
Species reactivity

Reacts with: Mouse, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
ab247595 is the carrier-free version of ab92729 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247595 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Affinity purified
Clonality

Monoclonal
Clone number

EPR3822
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3822] - BSA and Azide free (ab247595)

This data was developed using ab92729, the same antibody clone in a different buffer formulation.ab92729, at 1/50 dilution, staining PCNA in paraffin embedded Human colonic adenocarcinoma tissue by Immunohistochemisty. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3822] - BSA and Azide free (ab247595)

This data was developed using ab92729, the same antibody clone in a different buffer formulation.ab92729, at 1/50 dilution, staining PCNA in paraffin embedded Human tonsil tissue by Immunohistochemisty. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-PCNA antibody [EPR3822] - BSA and Azide free (ab247595)

All lanes : Anti-PCNA antibody [EPR3822] (ab92729) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 29 kDa

This data was developed using ab92729, the same antibody clone in a different buffer formulation.
Anti-PCNA antibody [EPR3822] - BSA and Azide free (ab247595)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com