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Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6577] to Cytochrome p450 2C19 - C-terminal
  • Suitable for: WB, Flow Cyt, IP, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal
    See all Cytochrome p450 2C19 primary antibodies

  • Description

    Rabbit monoclonal [EPR6577] to Cytochrome p450 2C19 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa cells, 293 cells, Human fetal liver tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)
    Flow Cytometry - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)

    Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling Cytochrome P450 2C19 with ab185213 at 1/240 (red) or Rabbit monoclonal IgG (green) followed by Goat anti rabbit IgG (FITC) at 1/150.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)

    Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells labeling Cytochrome P450 2C19 with ab185213 at 1/250 followed by Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 and counterstained with Dapi.

  • Immunoprecipitation - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)
    Immunoprecipitation - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)

    Immunoprecipitation analysis of 293 cell lysate immunoprecipitated for Cytochrome P450 2C19 using ab185213 at 1/50 dilution (Lane 1). A aAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution was used as secondary. Lane 2: PBS instead of 293 lysates

  • Western blot - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)
    Western blot - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)
    All lanes : Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213) at 1/5000 dilution

    Lane 1 : HeLa cell line lysate
    Lane 2 : 293 cell line lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)
    Western blot - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)
    Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213) at 1/1000 dilution + Human fetal liver lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?

  • Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)
    Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)

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