Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6577] to Cytochrome p450 2C19 - C-terminal
Suitable for: WB, Flow Cyt, IP, ICC/IF
Reacts with: Human

Product name

Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal
See all Cytochrome p450 2C19 primary antibodies
Description

Rabbit monoclonal [EPR6577] to Cytochrome p450 2C19 - C-terminal
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa cells, 293 cells, Human fetal liver tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6577
Isotype

IgG
Research areas

Flow Cytometry - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)

Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling Cytochrome P450 2C19 with ab185213 at 1/240 (red) or Rabbit monoclonal IgG (green) followed by Goat anti rabbit IgG (FITC) at 1/150.
Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)

Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells labeling Cytochrome P450 2C19 with ab185213 at 1/250 followed by Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 and counterstained with Dapi.
Immunoprecipitation - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)

Immunoprecipitation analysis of 293 cell lysate immunoprecipitated for Cytochrome P450 2C19 using ab185213 at 1/50 dilution (Lane 1). A aAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution was used as secondary. Lane 2: PBS instead of 293 lysates
Western blot - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)

All lanes : Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213) at 1/5000 dilution

Lane 1 : HeLa cell line lysate
Lane 2 : 293 cell line lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 56 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Western blot - Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)

Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213) at 1/1000 dilution + Human fetal liver lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 56 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Anti-Cytochrome p450 2C19 antibody [EPR6577] - C-terminal (ab185213)

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