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Anti-SPTLC3 antibody (ab237532)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-SPTLC3 antibody (ab237532)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to SPTLC3
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SPTLC3 antibody
    See all SPTLC3 primary antibodies
  • Description

    Rabbit polyclonal to SPTLC3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human SPTLC3 aa 1-100.
    Database link: Q9NUV7

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Mouse liver and kidney lysates; Rat heart and kidney lysates; U-87 MG, HepG2, HEK-293T and A549 whole cell lysates. IHC-P: Human liver cancer tissue. ICC/IF: HepG2 cells.
  • General notes

     This product was previously labelled as Serine palmitoyltransferase 3

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity greater than 95%
  • Clonality

    Polyclonal
  • Isotype

    IgG

Images

  • Western blot - Anti-SPTLC3 antibody (ab237532)
    Western blot - Anti-SPTLC3 antibody (ab237532)
    All lanes : Anti-SPTLC3 antibody (ab237532) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution

    Predicted band size: 62 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTLC3 antibody (ab237532)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTLC3 antibody (ab237532)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining human prostate cancer using ab237532 at 1/100. 
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTLC3 antibody (ab237532)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTLC3 antibody (ab237532)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining human colon cancer using ab237532 at 1/100. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTLC3 antibody (ab237532)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTLC3 antibody (ab237532)

    Paraffin-embedded human liver cancer tissue stained for SPTLC3 using ab237532 at 1/300 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

  • Immunocytochemistry/ Immunofluorescence - Anti-SPTLC3 antibody (ab237532)
    Immunocytochemistry/ Immunofluorescence - Anti-SPTLC3 antibody (ab237532)

    HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for SPTLC3 (green) using ab237532 at 1/100 dilution in ICC/IF. Secondary antibody is an Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).

  • Western blot - Anti-SPTLC3 antibody (ab237532)
    Western blot - Anti-SPTLC3 antibody (ab237532)
    All lanes : Anti-SPTLC3 antibody (ab237532) at 1/1000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 4 : Rat heart tissue lysate
    Lane 5 : Rat kidney tissue lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 62 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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