Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-FAF1 antibody [EPR14754] (ab183045)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14754] to FAF1
Suitable for: IHC-P, WB, ICC/IF, IP, Flow Cyt
Reacts with: Human

Product name

Anti-FAF1 antibody [EPR14754]
See all FAF1 primary antibodies
Description

Rabbit monoclonal [EPR14754] to FAF1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- THP1, 293, Hela and HT29 lysates; Human colon tissue; HeLa and 293 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14754
Isotype

IgG
Research areas

Western blot - Anti-FAF1 antibody [EPR14754] (ab183045)

All lanes : Anti-FAF1 antibody [EPR14754] (ab183045) at 1/50000 dilution

Lane 1 : THP1 cell lysate
Lane 2 : 293 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HT29 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 74 kDa
Observed band size: 74 kDa
Immunoprecipitation - Anti-FAF1 antibody [EPR14754] (ab183045)

Western blot analysis of FAF1 in 293 lysate immunoprecipitated with ab183045 at 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody used at 1/1000 dilution.
Flow Cytometry - Anti-FAF1 antibody [EPR14754] (ab183045)

Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling FAF1 with ab183045 at 1/200 dilution (pink) compared to a Rabbit monoclonal IgG isotype control (green) followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 diution.
Immunocytochemistry/ Immunofluorescence - Anti-FAF1 antibody [EPR14754] (ab183045)

Immunofluorescent analysis of acetone-fixed HeLa cells labeling FAF1 with ab183045 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAF1 antibody [EPR14754] (ab183045)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling FAF1 with ab183045 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-FAF1 antibody [EPR14754] (ab183045)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com