Anti-FAF1 antibody (ab202298)
Key features and details
- Rabbit polyclonal to FAF1
- Suitable for: WB, ICC/IF, IP, Flow Cyt
- Reacts with: Mouse, Rat, Chicken, Human, Xenopus laevis
- Isotype: IgG
Overview
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Product name
Anti-FAF1 antibody
See all FAF1 primary antibodies -
Description
Rabbit polyclonal to FAF1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IP, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Chicken, Human, Xenopus laevis -
Immunogen
Recombinant fragment corresponding to Human FAF1 aa 1-300.
Database link: Q9UNN5 -
Positive control
- Xenopus laevis crude egg extracts; U2OS extracts; U2OS cells transfected with HA-FAF1; U2OS cells; U2OS cell lysate; NIH3T3, INS1 and DT40 cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FAF1 antibody (ab202298)All lanes : Anti-FAF1 antibody (ab202298) at 1/2000 dilution
Lane 1 : U2OS cell lysate transfected with Control siRNA
Lane 2 : U2OS cell lysate transfected with FAF1 siRNA
Lane 3 : NIH3T3 cell lysate
Lane 4 : INS1 cell lysate
Lane 5 : DT40 cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 74 kDa
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Western blot - Anti-FAF1 antibody (ab202298)All lanes : Anti-FAF1 antibody (ab202298) at 1/2000 dilution
Lane 1 : Xenopus laevis crude egg extracts
Lane 2 : U2OS extracts
Lysates/proteins at 50 µg per lane.
Predicted band size: 74 kDa
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Immunoprecipitation - Anti-FAF1 antibody (ab202298)Western blot analysis with ab202298 immunoprecipitation using U2OS cell extracts.
Lane 1: Input.
Lane 2: Immunoprecipitation with control serum.
Lane 3: Immunoprecipitation with ab202298 (5 µL).
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Immunocytochemistry/ Immunofluorescence - Anti-FAF1 antibody (ab202298)Immunofluorescent analysis of PFA fixed, methanol permeabilized U2OS cells transfected with HA-FAF1 labeling FAF1 with ab202298 at 1/600 dilution (red). HA (green).
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Flow Cytometry - Anti-FAF1 antibody (ab202298)Flow cytometric analysis of U2OS cells labelling FAF1 with ab202298 at 1/200 dilution. Labeling with IgG control (upper panel), ab202298 in Control siRNA-treated cells (middle panel) or ab202298 in FAF1 siRNA treated cells (lower panel) is shown. Cells were fixed with 1% PFA. Cells were incubated with the primary antibody overnight at 4°C.
