Protein A Agarose (ab193254)
Key features and details
- Sample type: Ascites Fluid, Cell culture media, Cell culture supernatant, Serum
Overview
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Product name
Protein A Agarose -
Sample type
Cell culture supernatant, Serum, Cell culture media, Ascites Fluid -
Product overview
High binding capacity (>20 mg IgG/mL). Minimal leaching of ligand. Suitable for column or batch purification of IgG, immunoprecipitation & ChIP (ab193254).
Contents:
Supplied as a 50% slurry in 20% Ethanol.
Features:
High binding capacity = Binding of IgG = 20 mg human or rabbit IgG/mL Protein A Agarose.
Minimal leaching of the ligand
Flow Rate Tested* = 2.89 mL/min.
*Test condition: = Calculations based on the time required to pass 18 mL of water through 2 mL settled beads (column diameter 1.5 cm).
Usage = Reusable for up to 10 times without significant loss of binding capacity.Store beads at 4°C.
The beads may be damaged above 40°C.
DO NOT FREEZE.
Wash beads 3 times with 3x bead volume of desired buffer before use.
Applications:
- Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
- Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen. -
Notes
Protein A Agarose beads are prepared by covalently coupling recombinant Protein A to 6% cross-linked Agarose beads, the most popular resin for protein affinity purification methods. Protein A is a genetically engineered protein containing five IgG-binding regions of native Protein A. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein A to ensure maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG and minimum leaching of recombinant Protein A compared to standard Protein A agarose beads. The IgG binding capacity of Protein A Agarose is = 20 mg human or rabbit IgG per mL of wet beads. Protein A Agarose beads display high chemical and physical stability as well as high flow rate, hydrophilicity and high gel strength. This product can be used for IgG purification and immunoprecipitation.
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Tested applications
Suitable for: IP, Purificationmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 ml 5 ml 25 ml 100 ml Protein A-Agarose 1 x 1ml 1 x 5ml 1 x 25ml 1 x 100ml -
Research areas
