Human DcR3 ELISA Kit (ab242244)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 42 pg/ml
- Range: 63 pg/ml - 4000 pg/ml
- Sample type: EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human DcR3 ELISA Kit
See all DcR3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% plasma 8 2.7% Inter-assay Sample n Mean SD CV% plasma 3 7.5% -
Sample type
Serum, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
42 pg/ml -
Range
63 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 87 82% - 97% EDTA Plasma 112 108% - 115% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human DcR3 ELISA Kit (ab242244) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DcR3 protein in edta plasma and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human DcR3 with 42 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human DcR3 Capture Antibody 1 x 600µl 10X Human DcR3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR 1 x 6ml Human DcR3 Lyophilized recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Decoy receptor for the cytotoxic ligands TNFS14/LIGHT and TNFSF6/FASL. Protects against apoptosis. -
Tissue specificity
Detected in fetal lung, brain and liver. Detected in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung. Highly expressed in several primary tumors from colon, stomach, rectum, esophagus and in SW480 colon carcinoma cells. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- DcR3
- Decoy receptor 3
- Decoy receptor for Fas ligand
see all -
Database links
- Entrez Gene: 8771 Human
- Omim: 603361 Human
- SwissProt: O95407 Human
- Unigene: 434878 Human
Images
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Other - Human DcR3 ELISA Kit (ab242244)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human DcR3 standard curve in Sample Diluent NS.The DcR3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of spiked DcR3 in human plasma (EDTA) and serum samples.The concentrations of DcR3 were measured in duplicates, interpolated from the DcR3 standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (EDTA) 100% The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). -
Sandwich ELISA - Human DcR3 ELISA Kit (ab242244)To learn more about the advantages of recombinant antibodies see here.
