Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human DcR3 ELISA Kit (ab242244)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 42 pg/ml
  • Range: 63 pg/ml - 4000 pg/ml
  • Sample type: EDTA Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human DcR3 ELISA Kit
    See all DcR3 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    plasma 8 2.7%
    Inter-assay
    Sample n Mean SD CV%
    plasma 3 7.5%

  • Sample type

    Serum, EDTA Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    42 pg/ml

  • Range

    63 pg/ml - 4000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 87 82% - 97%
    EDTA Plasma 112 108% - 115%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human DcR3 ELISA Kit (ab242244) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DcR3 protein in edta plasma and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human DcR3 with 42 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human DcR3 Capture Antibody 1 x 600µl
    10X Human DcR3 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPR 1 x 6ml
    Human DcR3 Lyophilized recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Decoy receptor for the cytotoxic ligands TNFS14/LIGHT and TNFSF6/FASL. Protects against apoptosis.

  • Tissue specificity

    Detected in fetal lung, brain and liver. Detected in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung. Highly expressed in several primary tumors from colon, stomach, rectum, esophagus and in SW480 colon carcinoma cells.

  • Sequence similarities

    Contains 4 TNFR-Cys repeats.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • DcR3
    • Decoy receptor 3
    • Decoy receptor for Fas ligand
    • DJ583P15.1.1
    • M68
    • M68E
    • OTTHUMP00000031583
    • TNF6B_HUMAN
    • TNFRSF6B
    • TR6
    • Tumor necrosis factor receptor superfamily member 6B
    • Tumor necrosis factor receptor superfamily member 6b decoy
    • UNQ186/PRO212
    see all

  • Database links

    Images

    • Other - Human DcR3 ELISA Kit (ab242244)
      Other - Human DcR3 ELISA Kit (ab242244)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of human DcR3 standard curve in Sample Diluent NS.
      Example of human DcR3 standard curve in Sample Diluent NS.
      The DcR3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
    • Interpolated concentrations of spiked DcR3 in human plasma (EDTA) and serum samples.
      Interpolated concentrations of spiked DcR3 in human plasma (EDTA) and serum samples.
      The concentrations of DcR3 were measured in duplicates, interpolated from the DcR3 standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (EDTA) 100% The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
    • Sandwich ELISA - Human DcR3 ELISA Kit (ab242244)
      Sandwich ELISA - Human DcR3 ELISA Kit (ab242244)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Alternative products to Human DcR3 ELISA Kit (ab242244)

    © 2021 Astana Biomed Group LLP. All rights reserved.