Human DcR3 ELISA Kit (ab193697)
Key features and details
- Sensitivity: 0.3 ng/ml
- Range: 0.3 ng/ml - 80 ng/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human DcR3 ELISA Kit
See all DcR3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
0.3 ng/ml -
Range
0.3 ng/ml - 80 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 129.9 107% - 144% Plasma 107.5 74% - 134% Cell culture media 141.9 122% - 150% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam's DcR3 Human ELISA Kit (ab193697) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human DcR3 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for human DcR3 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures DcR3 present in the samples. The wells are washed and biotinylated anti-human DcR3 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of DcR3 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated Human DcR3 detection antibody (lyophilized) 2 vials Human DcR3 Standards (lyophilized) 2 vials Pre-coated Human DcR3 Microplate (12 Strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Function
Decoy receptor for the cytotoxic ligands TNFS14/LIGHT and TNFSF6/FASL. Protects against apoptosis. -
Tissue specificity
Detected in fetal lung, brain and liver. Detected in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung. Highly expressed in several primary tumors from colon, stomach, rectum, esophagus and in SW480 colon carcinoma cells. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- DcR3
- Decoy receptor 3
- Decoy receptor for Fas ligand
see all -
Database links
- Entrez Gene: 8771 Human
- Omim: 603361 Human
- SwissProt: O95407 Human
- Unigene: 434878 Human
Images
-
Example Standard Curve
