Anti-Wnt7a antibody [EPR23471-125] - BSA and Azide free (ab274324)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23471-125] to Wnt7a - BSA and Azide free
- Suitable for: WB, ICC
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Wnt7a antibody [EPR23471-125] - BSA and Azide free
See all Wnt7a primary antibodies -
Description
Rabbit monoclonal [EPR23471-125] to Wnt7a - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICCmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HaCaT and BxPC-3 whole cell lysates; Mouse P7 hippocampus tissue lysate. ICC: HaCaT cells.
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General notes
ab274324 is the carrier-free version of ab274321. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23471-125 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Wnt7a antibody [EPR23471-125] (ab274321) at 1/1000 dilution
Lane 1 : HaCaT (human skin keratinocyte) whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : BxPC-3 (human pancreas adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : Mouse P7 hippocampus tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))
Lanes 2-4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 39 kDa
Observed band size: 42/40 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 25978974, 30143647, 30770219).
Negative control: HeLa cells (PMID: 25978974).
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 minutes
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab274321)
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HaCaT and HeLa cells labelling Wnt7a with ab274321 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong cytoplasmic and weak nuclear staining in HaCaT cells. Low expression cell line: HeLa (PMID: 25978974). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at a 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab274321).
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