Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8080] to Nucleolin (phospho T84)
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Nucleolin (phospho T84) antibody [EPR8080]
See all Nucleolin primary antibodies -
Description
Rabbit monoclonal [EPR8080] to Nucleolin (phospho T84) -
Host species
Rabbit -
Specificity
ab155977 only detects Nucleolin phosphorylated at threonine 84. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human Nucleolin (phospho T84). The exact sequence is proprietary.
Database link: P19338 -
Positive control
- 293T cell lysate; ovarian carcinoma and tonsil tissues.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8080 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977) at 1/1000 dilution
Lane 1 : 293T cell lysate, untreated
Lane 2 : 293T cell lysate treated with Lambda Phosphatase
Lysates/proteins at 10 µg per lane.
Predicted band size: 77 kDa
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Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue, labeling Nucleolin using ab155977 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling Nucleolin using ab155977 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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