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Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)

Price and availability

311 587 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8080] to Nucleolin (phospho T84)
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-Nucleolin (phospho T84) antibody [EPR8080]
    See all Nucleolin primary antibodies
  • Description

    Rabbit monoclonal [EPR8080] to Nucleolin (phospho T84)
  • Host species

    Rabbit
  • Specificity

    ab155977 only detects Nucleolin phosphorylated at threonine 84.
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Nucleolin (phospho T84). The exact sequence is proprietary.
    Database link: P19338

  • Positive control

    • 293T cell lysate; ovarian carcinoma and tonsil tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8080
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nucleolus
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other

Images

  • Western blot - Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)
    Western blot - Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)
    All lanes : Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977) at 1/1000 dilution

    Lane 1 : 293T cell lysate, untreated
    Lane 2 : 293T cell lysate treated with Lambda Phosphatase

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 77 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)

    Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue, labeling Nucleolin using ab155977 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling Nucleolin using ab155977 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)
    Anti-Nucleolin (phospho T84) antibody [EPR8080] (ab155977)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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