Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8080-2] to Nucleolin (phospho T76)
Suitable for: WB, IHC-P, IP
Reacts with: Human

Product name

Anti-Nucleolin (phospho T76) antibody [EPR8080-2]
See all Nucleolin primary antibodies
Description

Rabbit monoclonal [EPR8080-2] to Nucleolin (phospho T76)
Host species

Rabbit
Specificity

ab168363 only detects Nucleolin when phosphorylated at threonine 76.

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human Nucleolin.
Positive control
- 293T cell lysates; Human tonsil tissue and urinary bladder transitional cell carcinoma tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR8080-2
Isotype

IgG
Research areas

Western blot - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

All lanes : Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363) at 1/1000 dilution

Lane 1 : 293T cell lysates, untreated
Lane 2 : 293T cell lysates, treated with Lambda Phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 77 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

ab168363 showing +ve staining in Human ovarian carcinoma.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

ab168363 showing +ve staining in Human kidney carcinoma.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

ab168363 showing +ve staining in Human breast carcinoma.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

ab168363 showing +ve staining in Human endometrial carcinoma.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Nucleolin with ab168363 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

Immunohistochemical analysis of paraffin-embedded Human urinary bladder transitional cell carcinoma tissue labeling Nucleolin with ab168363 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

Western blot analysis on immunoprecipitation pellet from 293 cell lysate using anti Phospho Nucleolin (pT76) ab158363 at 1/10 dilution.
Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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