Anti-Nucleolin antibody [SPM614] - BSA and Azide free (ab212671)
![Anti-Nucleolin antibody [SPM614] - BSA and Azide free (ab212671)](https://www.abcam.com/ps/products/212/ab212671/Images/ab212671-3-anti-nucleolin-antibody-spm614-immunocytochemistry-k562-human.jpg "Anti-Nucleolin antibody [SPM614] - BSA and Azide free (ab212671)")
Key features and details
- Mouse monoclonal [SPM614] to Nucleolin - BSA and Azide free
- Suitable for: IHC-P, ICC
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Nucleolin antibody [SPM614] - BSA and Azide free
See all Nucleolin primary antibodies -
Description
Mouse monoclonal [SPM614] to Nucleolin - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, ICCmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat, Cow -
Immunogen
Recombinant full length protein corresponding to Human Nucleolin.
Database link: P19338 -
Positive control
- IHC-P: Human tonsil tissue. ICC: HeLa and K562 cells.
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General notes
ab212671 can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from bioreactor concentrate. -
Clonality
Monoclonal -
Clone number
SPM614 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry - Anti-Nucleolin antibody [SPM614] - BSA and Azide free (ab212671)Immunocytochemistry analysis of K562 (Human chronic myelogenous leukemia lymphoblast cell line) cells labeling Nucleolin with ab212671 at 1 ug/ml follower by goat anti-mouse IgG-CF488 (green). Membrane stained with phalloidin-CF640 (red)
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![Immunocytochemistry - Anti-Nucleolin antibody [SPM614] - BSA and Azide free (ab212671)](https://www.abcam.com/ps/products/212/ab212671/Images/ab212671-2-anti-nucleolin-antibody-spm614-immunocytochemistry-hela-human.jpg)
Immunocytochemistry - Anti-Nucleolin antibody [SPM614] - BSA and Azide free (ab212671)Immunocytochemistry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Nucleolin with ab212671 at 1 ug/ml (green). Nuclei stained with DAPI (blue). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [SPM614] - BSA and Azide free (ab212671)](https://www.abcam.com/ps/products/212/ab212671/Images/ab212671-263896-anti-nucleolin-antibody-spm614-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [SPM614] - BSA and Azide free (ab212671)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue section labeling Nucleolin with ab212671 at 1 µg/mL.
