Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Nucleolin antibody [EPR7952] (ab129200)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7952] to Nucleolin
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-Nucleolin antibody [EPR7952]
See all Nucleolin primary antibodies
Description

Rabbit monoclonal [EPR7952] to Nucleolin
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Rat Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Nucleolin aa 650-750 (C terminal). The exact sequence is proprietary.
Positive control
- WB: Fetal brain tissue lysate and K562, and HuT-78 cell lysates. IHC-P: Human colon, mouse liver and rat liver tissues. Flow Cyt: HeLa cells. ICC/IF: HT-29
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 5.80 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR7952
Isotype

IgG
Research areas

Western blot - Anti-Nucleolin antibody [EPR7952] (ab129200)

All lanes : Anti-Nucleolin antibody [EPR7952] (ab129200) at 1/10000 dilution

Lane 1 : Fetal brain lysate
Lane 2 : K562 cell lysate
Lane 3 : HuT-78 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 76 kDa
Observed band size: 100 kDa
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-Nucleolin antibody [EPR7952] (ab129200)

Immunocytochemistry/ Immunofluorescence analysis of HT-29(Human colorectal adenocarcinoma epithelial cell) cells labeling Nucleolin with purified ab129200 at 1/500 dilution (4.5 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [EPR7952] (ab129200)

ab129200, at 1/250 dilution, staining Nucleolin in paraffin-embedded Human colon tissue by Immunohistochemistry.
Flow Cytometry - Anti-Nucleolin antibody [EPR7952] (ab129200)

Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Nucleolin (red) with ab129200 at a 1/2000 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [EPR7952] (ab129200)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling Nucleolin with ab129200 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A ready to use HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [EPR7952] (ab129200)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling Nucleolin with ab129200 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A ready to use HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Counterstained with hematoxylin.
OI-RD Scanning - Anti-Nucleolin antibody [EPR7952] (ab129200)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-Nucleolin antibody [EPR7952] (ab129200)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com