Alexa Fluor® 488 Anti-Nucleolin antibody [364-5] (ab154028)
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [364-5] to Nucleolin
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG1
Overview
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Product name
Alexa Fluor® 488 Anti-Nucleolin antibody [364-5]
See all Nucleolin primary antibodies -
Description
Alexa Fluor® 488 Mouse monoclonal [364-5] to Nucleolin -
Host species
Mouse -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Tissue, cells or virus corresponding to Human Nucleolin.
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Positive control
- HDFn and HeLa cells.
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General notes
Product was previously marketed under the MitoSciences sub-brand.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab154028 is purified by biochemical fractionation. Near homogeneity as judged by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
364-5 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry with HeLa cells were stained with Anti-Nucleolin-488 antibody (0.2 µg/mL, ab154028) in green, Anti-GAPDH (1.0 µg/mL, ab110305) as red, and DAPI (1/10,000) in blue, as a nuclear stain. Secondary antibody used was goat anti-mouse IgG1 Alexa-594 at 1/1000 dilution. A) HeLa cells 40x. B) HeLa cells 100x.
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Immunocytochemistry with HDFn cells were stained with Anti-Nucleolin-488 antibody (0.2 µg/mL, ab154028) in green, Anti-GAPDH (1.0 µg/mL, ab110305) as red, and DAPI in blue, as a nuclear stain. Secondary antibody used was goat anti-mouse IgG1 Alexa-594 at 1/1000 dilution. A) HDFn 40x. B) HDFn 100x.
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Flow Cytometry for Anti-Nucleolin-488 antibody (ab154028) stained HeLa cells. Flow cytometry results of unstained cells (black) and 1.0 µg/mL Nucleolin-488 (red) stained cells.