Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Thursday March 04, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4102] to CD30
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-CD30 antibody [EPR4102]
See all CD30 primary antibodies
Description

Rabbit monoclonal [EPR4102] to CD30
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

corresponding to Human CD30 (C terminal).
Database link: P28908
Positive control
- IHC-P: Human Hodgkin's lymphoma and human tonsil tissue WB: KARPAS-299 whole cell lysate; HDLM-2 whole cell lysate
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4102
Isotype

IgG
Research areas

Western blot - Anti-CD30 antibody [EPR4102] (ab134080)

All lanes : Anti-CD30 antibody [EPR4102] (ab134080) at 1/200 dilution (purified)

Lane 1 : KARPAS-299 (Human anaplastic large cell lymphoma) whole cell lysates
Lane 2 : HDLM-2 (Human Hodgkin lymphoma) whole cell lysates
Lane 3 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
Lane 4 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Developed using the ECL technique.

Predicted band size: 64 kDa
Observed band size: 120,90 kDa
why is the actual band size different from the predicted?

Exposure time: 60 seconds

The 90 KD band is precursor and 120 KD is the mature transmembrane form as is described in PMID: 11120787.
Raji and Daudi are negative for CD30 as is described in PMID: 29316337.
This antibody detects high background.

Blocking buffer and concentration:5% NFDM/TBST

Diluting buffer and concentration:2% BSA/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [EPR4102] (ab134080)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD30 with purified ab134080 at 1/2000 dilution (0.82 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [EPR4102] (ab134080)

Immunohistochemical analysis of paraffin embedded Human Hodgkin's lymphoma tissue labelling CD30 with ab134080 (unpurified) antibody at a dilution of 1/1000. Heat mediated antigen retrieval was perfomed before commencing with IHC staining protocol.
Anti-CD30 antibody [EPR4102] (ab134080)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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