Anti-EGFR (phospho Y1148) antibody (ab47364)
Key features and details
- Rabbit polyclonal to EGFR (phospho Y1148)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EGFR (phospho Y1148) antibody
See all EGFR primary antibodies -
Description
Rabbit polyclonal to EGFR (phospho Y1148) -
Host species
Rabbit -
Specificity
Recognises EGFR phosphorylated on Tyrosine 1148 of the mature human isoform 1 (corresponding to Y1148 from the precursor form P00533-1/p170) -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human EGFR (phospho Y1148).
Sequence:PD-YP-QQ
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Positive control
- Extract of EGF treated A431 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EGFR (phospho Y1148) antibody (ab47364)All lanes : Anti-EGFR (phospho Y1148) antibody (ab47364) at 1/500 dilution
Lane 1 : Extract of A431 cells treated with 40µM EGF for 10 min
Lane 2 : Extract of A431 cells
Predicted band size: 134 kDa
Typically 5-30ug of total protein was loaded per lane of the gel.
