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PerCP Anti-Integrin beta 1 antibody [P5D2] (ab219738)

Key features and details

  • PerCP Mouse monoclonal [P5D2] to Integrin beta 1
  • Suitable for: Flow Cyt
  • Knockout validated
  • Reacts with: Human
  • Conjugation: PerCP. Ex: 482nm, Em: 675nm
  • Isotype: IgG1

Overview

  • Product name

    PerCP Anti-Integrin beta 1 antibody [P5D2]
    See all Integrin beta 1 primary antibodies

  • Description

    PerCP Mouse monoclonal [P5D2] to Integrin beta 1

  • Host species

    Mouse

  • Conjugation

    PerCP. Ex: 482nm, Em: 675nm

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus. Fibroblast cells (HT1080).

  • Positive control

    • Flow Cyt: MCF7 cells. Live HAP1-WT cells.

  • General notes

    This antibody inhibits the function of all beta 1 Integrins.

Properties

Images

  • Flow Cytometry - PerCP Anti-Integrin beta 1 antibody [P5D2] (ab219738)
    Flow Cytometry - PerCP Anti-Integrin beta 1 antibody [P5D2] (ab219738)
    Overlay histogram showing HAP1 wildtype (green line) and HAP1-ITGB1 knockout cells (red line) stained with ab219738. Live HAP1 wildtype and HAP1-ITGB1 knockout cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab219738, 10 µg/ml) for 30 min at 4°C.
    A mouse IgG1 isotype control antibody (IC002C, R&D systems)  was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-ITGB1 knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 685/35 bandpass filter.
  • Flow Cytometry - PerCP Anti-Integrin beta 1 antibody [P5D2] (ab219738)
    Flow Cytometry - PerCP Anti-Integrin beta 1 antibody [P5D2] (ab219738)

    Overlay histogram showing MCF7 cells stained with ab219738 (red line). The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab219738, 1/50 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) PerCP (ab222107) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 685/35 bandpass filter.

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