Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Dlx5 antibody [EPR4488] - BSA and Azide free (ab239987)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4488] to Dlx5 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Dlx5 antibody [EPR4488] - BSA and Azide free
    See all Dlx5 primary antibodies

  • Description

    Rabbit monoclonal [EPR4488] to Dlx5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab239987 is the carrier-free version of ab109737. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab239987 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Dlx5 antibody [EPR4488] - BSA and Azide free (ab239987)
    Immunocytochemistry/ Immunofluorescence - Anti-Dlx5 antibody [EPR4488] - BSA and Azide free (ab239987)

    Immunocytochemistry/Immunofluorescence analysis of C6 (rat glioma) cells labelling Dlx5 with purified ab109737 at 1/1000. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109737).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dlx5 antibody [EPR4488] - BSA and Azide free (ab239987)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dlx5 antibody [EPR4488] - BSA and Azide free (ab239987)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human astrocytoma tissue labelling Dlx5 with purified ab109737 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109737).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dlx5 antibody [EPR4488] - BSA and Azide free (ab239987)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dlx5 antibody [EPR4488] - BSA and Azide free (ab239987)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human brain tissue labelling Dlx5 with unpurified ab109737 at 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109737).

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Western blot - Anti-Dlx5 antibody [EPR4488] - BSA and Azide free (ab239987)
    Western blot - Anti-Dlx5 antibody [EPR4488] - BSA and Azide free (ab239987)
    Lane 1 : Anti-Dlx5 antibody [EPR4488] (ab109737) at 1/1000 dilution
    Lane 2 : Anti-GST antibody [EPR4236] - BSA and Azide free (ab235384) at 1/1000 dilution
    Lane 3 : Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) at 1/1000 dilution

    All lanes : GST and His tagged recombinant Human Dlx5 protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 32 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    This data was developed using ab109737, the same antibody clone in a different buffer formulation.

  • Anti-Dlx5 antibody [EPR4488] - BSA and Azide free (ab239987)
    Anti-Dlx5 antibody [EPR4488] - BSA and Azide free (ab239987)

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