Antibodies, Proteins, Kits and Reagents for Life Science

Anti-CD45 antibody [BL-178-12C7] (ab243869)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BL-178-12C7] to CD45
Suitable for: WB, IHC-P, ICC, IP
Reacts with: Human

Product name

Anti-CD45 antibody [BL-178-12C7]
See all CD45 primary antibodies
Description

Rabbit monoclonal [BL-178-12C7] to CD45
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human CD45 aa 1250-1306 (C terminal). The exact sequence is proprietary. NP_002829.3 and Gene ID 5788.
Database link: P08575
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BL-178-12C7
Isotype

IgG
Research areas

Immunoprecipitation - Anti-CD45 antibody [BL-178-12C7] (ab243869)

CD45 was immunoprecipitated from 1 mg Jurkat whole cell lysate with ab243869 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243869 at 1/1000 dilution.
Lane 1: ab243869 IP in Jurkat cell lysate.

Lane 2: Contol IgG in Jurkat cell lysate.

Detection: Chemiluminescence with an exposure time of 3 seconds.
Western blot - Anti-CD45 antibody [BL-178-12C7] (ab243869)

Western blot analysis using ab243869 at 1/1000 dilution.
Lane 1: Jurkat whole cell lysate (50 µg).
Lane 2: MOLT-4 whole cell lysate (50 µg).
Lane3: K562 whole cell lysate (50 µg).
Lane 4: SR whole cell lysate (50 µg).
Lane 5: HeLa whole cell lysate (50 µg).
Lane 6: HEK-293T whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 3 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [BL-178-12C7] (ab243869)

Formalin-fixed, paraffin-embedded human small intestine tissue stained for CD45 using ab243869 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
Immunocytochemistry - Anti-CD45 antibody [BL-178-12C7] (ab243869)

Formaldehyde-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling CD45 using ab243869 at 1/250 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [BL-178-12C7] (ab243869)

Formalin-fixed, paraffin-embedded human appendix tissue stained for CD45 using ab243869 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
Anti-CD45 antibody [BL-178-12C7] (ab243869)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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