Anti-Vimentin (phospho S82) antibody [EP1071Y] (ab52943)
![Anti-Vimentin (phospho S82) antibody [EP1071Y] (ab52943)](https://www.abcam.com/ps/products/52/ab52943/Images/ab52943-256530-anti-vimentin-phospho-s82-antibody-ep1071y-dot-blot.jpg "Anti-Vimentin (phospho S82) antibody [EP1071Y] (ab52943)")
Key features and details
- Rabbit monoclonal [EP1071Y] to Vimentin (phospho S82)
- Suitable for: Dot blot, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Vimentin (phospho S82) antibody [EP1071Y]
See all Vimentin primary antibodies -
Description
Rabbit monoclonal [EP1071Y] to Vimentin (phospho S82) -
Host species
Rabbit -
Specificity
ab52943 only detects Vimentin phosphorylated at Serine 82. -
Tested applications
Suitable for: Dot blot, WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Vimentin (phospho S82). The exact sequence is proprietary.
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Positive control
- WB: AP treated and untreated HeLa + Calyculin A cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1071Y -
Isotype
IgG -
Research areas
Images
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Dot Blot - Anti-Vimentin (phospho S82) antibody [EP1071Y] (ab52943)
Primary antibody dilution: 1/1000
Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated
Secondary antibody dilution: 1/2500
Blocking & dilution buffer: 5% NFDM/TBST
Lane 1 sample: Vimentin (pS82) phospho peptide
Lane 2 sample: Vimentin non-phospho peptide
Exposure time: 3 minutes
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![Western blot - Anti-Vimentin (phospho S82) antibody [EP1071Y] (ab52943)](https://www.abcam.com/ps/products/52/ab52943/Images/ab52943-65463-anti-vimentin-phospho-s82-antibody-ep1071y-western-blot.gif)
Western blot - Anti-Vimentin (phospho S82) antibody [EP1071Y] (ab52943)All lanes : Anti-Vimentin (phospho S82) antibody [EP1071Y] (ab52943) at 1/500000 dilution
Lane 1 : HeLa cell lysate AP treated
Lane 2 : HeLa cell lysate (AP untreated) + Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
