Anti-Metallothionein antibody (ab36882)
Key features and details
- Rabbit polyclonal to Metallothionein
- Suitable for: ELISA, WB
- Isotype: IgG
Overview
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Product name
Anti-Metallothionein antibody
See all Metallothionein primary antibodies -
Description
Rabbit polyclonal to Metallothionein -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with Atlantic cod (Gadus morhua) and with metallothionein from a wide variety of different species including whiting (Merlangius merlangus), yellow gurnard (Trigla lucerna) and flounder (Platichthys flesus) as well as some cypriniform, siluriform, salmoniform, perciform and rajiform species. -
Immunogen
Metallothionein purified from liver of Atlantic cod (Gadus morhua).
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Positive control
- WB: Liver S9 fractions.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 1.64% Sodium phosphate, 1% BSA, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ELISA - Anti-Metallothionein antibody (ab36882)Ab36882, at a concentration of 1 µg/ml, staining Metallothionein by ELISA. Coating buffer was used as a negative control.
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Western blot - Anti-Metallothionein antibody (ab36882)All lanes : Anti-Metallothionein antibody (ab36882) at 1/500 dilution
Lane 2 : Purified metallothionein from Atlantic cod at 0.25 µg
Lane 3 : Liver S9 fractions from cadmium treated Atlantic cod at 2.5 µg
Lane 4 : Liver S9 fractions from cadmium treated Whiting at 2.5 µg
Lane 5 : Liver S9 fractions from cadmium treated Yellow gurnard at 2.5 µg
Lane 6 : Liver S9 fractions from cadmium treated Flounder at 2.5 µg
Predicted band size: 6 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Lane 1 represents the molecular weight standard.
